OXYGEN RADICAL PRODUCTION IS INCREASED IN MACROPHAGES FROM DIABETES-PRONE BB-RATS
OXYGEN RADICAL PRODUCTION IS INCREASED IN MACROPHAGES FROM DIABETES-PRONE BB-RATS
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DOI：
10.3109/08916939309043883
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发表时间：
1993-01-01
期刊：
影响因子：
3.5
通讯作者：
KOLB, H
中科院分区：
文献类型：
--
作者：
BRENNER, HH;BURKART, V;KOLB, H
研究发现，与糖尿病抗性 BB 或正常 Wistar 大鼠相比，自身免疫性糖尿病易感 BB 大鼠的巨噬细胞产生自由基氧中间体 (ROI) 的速度更高。 ROI 的释放通过化学发光测定，使用平行的鲁米诺和光泽精作为检测分子。在易患糖尿病的 BB 大鼠中，来自不同区室（即腹膜和脾脏）的巨噬细胞中 ROI 的自发释放上调。此外，对于来自易患糖尿病的 BB 大鼠的细胞，通过注射小棒杆菌体内或通过 LPS 和 IFN 体外激活巨噬细胞后，ROI 的最大输出最高。这种巨噬细胞异常在可识别的胰岛炎症之前就已在动物中出现，并且也存在于从易患糖尿病的 BB 大鼠的前体细胞体外生长的巨噬细胞水平上。氧自由基分泌过多可能导致 BB 大鼠的 β 细胞丢失和糖尿病的发生。
Macrophages from autoimmune diabetes prone BB rats were found to produce radical oxygen intermediates (ROI) at an enhanced rate when compared to diabetes resistant BB or normal Wistar rats. The release of ROI was determined by chemiluminescence using in parallel luminol and lucigenin as detector molecules. In diabetes prone BB rats the spontaneous release of ROI was upregulated in macrophages from different compartments, i.e. peritoneum and spleen. Also, maximal output of ROI after activation of macrophages either in vivo by injection of Corynebacterium parvum or in vitro by LPS and IFN was highest for cells from diabetes prone BB rats. This macrophage abnormality was seen in animals prior to recognizable islet inflammation and also was present at the level of macrophages grown in vitro from precursor cells of diabetes prone BB rats. Hypersecretion of oxygen radicals may contribute to Beta cell loss and diabetes development in BB rats.