Scanning electron microscopy of cloned astrocytic lines derived from ethylnitrosourea-induced rat gliomas
Scanning electron microscopy of cloned astrocytic lines derived from ethylnitrosourea-induced rat gliomas
复制标题
乙基亚硝基脲诱导的大鼠神经胶质瘤衍生的克隆星形细胞系的扫描电子显微镜
DOI：
10.1007/bf02889058
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
P. Coates
中科院分区：
文献类型：
--
作者：
A. Spence;P. Coates
本研究对4株乙基亚硝基脲诱导的大鼠F-344胶质瘤的6株星形细胞瘤细胞系进行了体外扫描电镜观察，并对2株星形细胞瘤细胞系进行了脑内移植后的体内观察。所有克隆均由单个胶质瘤细胞系内相当均一的星状细胞组成。所有肿瘤细胞系共有的细胞膜特征包括微绒毛、大泡、皱褶和微嵴，主要局限于胞体，丝状伪足主要来自细胞突起。一种细胞系表现出丰富的，另一种细胞系表现出中等程度的微绒毛发育和细胞表面粗糙化，这在一种肿瘤中与快速的体外细胞倍增时间相关。这些结果扩展了星形细胞瘤的扫描电镜观察，特别是在克隆的乙基亚硝基脲诱导的大鼠肿瘤中。他们证实，在这种类型的肿瘤中，细胞膜形貌确实发生了明显的变化，可能与其在人类或大鼠中的起源无关，也与其自发、化学诱导或病毒诱导肿瘤的发生模式无关。
SummarySix cloned astrocytoma cell lines derived from four ethylnitrosourea-induced F-344 rat gliomas were viewed by scanning electron microscopy in vitro, and two were examined in vivo after transplantation to the intracerebral site. All clones consisted of stellate cells that were reasonably homogeneous within individual glioma lines. Cell membrane features common to all tumor lines included microvilli, blebs, ruffles, and miniridges, mainly confined to perikarya, and filopodia emanating chiefly from cell processes. One cell line demonstrated a profuse, and another cell line a moderate, degree of microvillous development and cell surface roughening, which in one tumor correlated with rapid in vitro cell doubling time. Both cell lines maintained these topographical appearances when transplanted into brain.These results extend the SEM observations of astrocytomas, particularly in cloned ethylnitrosourea-induced tumors in rats. They confirm that distinct variations in cell membrane topography do occur among tumors of this type, probably irrespective of their origin in humans or rats, and irrespective of their mode of genesis as spontaneous, chemically-induced, or virallyinduced tumors.