Cellular Response to Metallic Ions Released from Nickel-Chromium Dental Alloys
Cellular Response to Metallic Ions Released from Nickel-Chromium Dental Alloys
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DOI：
10.1177/00220345950740081401
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发表时间：
1995-08
影响因子：
7.6
通讯作者：
J. Bumgardner;Linda C. Lucas
中科院分区：
文献类型：
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作者：
J. Bumgardner;Linda C. Lucas
由于镍铬牙科铸造合金对加速腐蚀的敏感性，镍和铍等金属离子可能会从牙科铸造合金中释放出来。在这项研究中，我们在为期三天的细胞培养试验中，评估了四种商业镍铬合金中金属离子的释放情况，这些合金代表了一系列的成分。原子吸收光谱分析表明，金属离子的释放与细胞形态、活性和增殖的变化有关。结果表明，试验合金及其腐蚀产物不影响细胞形态或存活率，但抑制细胞增殖。金属离子释放的类型和数量与合金的表面和腐蚀性能相对应，也与72 h后观察到的细胞增殖下降有关。与对照细胞相比，海王星对细胞增殖的影响最小，由于其耐蚀性、高铬钼含量、均匀的表面氧化物，释放的腐蚀产物数量最少。其他测试合金容易受到加速腐蚀过程的影响，释放出较高水平的金属离子，这与胸腺嘧啶核苷掺入的较大下降有关。所有合金的金属离子水平都随着试验时间的延长而增加，但与大块合金成分不成正比。Ni离子的释放量略高于大块合金成分，而Be离子的释放量是大块合金成分的4~6倍。Be离子释放的增加与细胞增殖减少有关。其他合金元素的释放水平与主体水平相似或低于主体水平。需要进一步的研究来评估释放的金属离子，特别是镍和铍离子，对细胞活动和功能可能产生的协同效应。
Concerns exist over the potential release of elevated levels of metal ions such as Ni and Be from Ni-Cr dental casting alloys, due to their susceptibility to accelerated corrosion. In this investigation, we evaluated the release of metal ions from four commercial Ni-Cr alloys, representing a range of compositions, in three-day cell culture tests. Metal ion release, as measured by atomic absorption spectroscopy, was correlated to changes in cellular morphology, viability, and proliferation. The results showed that the test alloys and their corrosion products did not affect cellular morphology or viabilities, but did decrease cellular proliferation. The types and amounts of metal ions released, which corresponded to the alloys' reported surface and corrosion properties, also correlated to observed decreases in cellular proliferation after 72 h. Neptune, which caused the smallest decrease in cellular proliferation as compared with control cells, released the lowest amount of corrosion products, due to its corrosion-resistant, high-Cr-Mo-containing, homogeneous surface oxide. The other test alloys, which were susceptible to accelerated corrosion processes, released higher levels of metal ions that correlated to larger decreases in thymidine incorporation. Metal ion levels increased with test time for all alloys but were not proportional to bulk alloy compositions. Ni ions were released at slightly higher than bulk alloy compositions, while Be was released at from four to six times that of bulk alloy compositions. The elevated release of Be ions was associated with reduced cellular proliferation. Other alloying elements were released at levels similar to or lower than bulk levels. Further research is needed to evaluate possible synergistic effects of released metal ions, especially Ni and Be ions, on cellular activities and functions.