Localization of sphingomyelin in cholesterol domains by imaging mass spectrometry
Localization of sphingomyelin in cholesterol domains by imaging mass spectrometry
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DOI：
10.1021/la063251f
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发表时间：
2007-05-08
期刊：
影响因子：
3.9
通讯作者：
Winograd, Nicholas
中科院分区：
文献类型：
--
作者：
McQuaw, Carolyn M.;Zheng, Leiliang;Winograd, Nicholas
在棕榈酰油酰磷脂酰胆碱/18:0鞘磷脂/胆固醇单层体系中，利用成像飞行时间二次离子质谱（TOF - SIMS）可在横向分辨出每种脂质的位置，而无需添加荧光标记。这种不相溶液相共存的体系所显示的胆固醇区域，其大小和形状与荧光显微镜文献中所描述的相当。结果表明，鞘磷脂（SM）与胆固醇共定位，而棕榈酰油酰磷脂酰胆碱被排斥在外。此外，这种分离并不完全，两种磷脂都有少量分布在各处。
The location of each lipid in a palmitoyloleoylphosphatidylcholine/18:0 sphingomyelin/cholesterol monolayer system is laterally resolved using imaging time-of-flight secondary ion mass spectrometry (TOF-SIMS) without the necessity of adding fluorescent labels. This system of coexisting immiscible liquid phases shows cholesterol domains with sizes and shapes comparable to those in the fluorescence microscopy literature. The results show that SM localizes with cholesterol and that palmitoyloleoylphosphatidylcholine is excluded. Moreover, the segregation is not complete, and there is a small amount of both phospholipids distributed throughout.