Evaluation of Burkholderia cepacia Complex Bacteria Pathogenicity Using Caenorhabditis elegans.
Evaluation of Burkholderia cepacia Complex Bacteria Pathogenicity Using Caenorhabditis elegans.
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DOI：
10.21769/bioprotoc.1964
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发表时间：
2016-10
期刊：
影响因子：
0.8
通讯作者：
P. Tedesco;E. Di Schiavi;F. Esposito;D. de Pascale
中科院分区：
文献类型：
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作者：
P. Tedesco;E. Di Schiavi;F. Esposito;D. de Pascale
本方案描述了两种评估洋葱伯克氏菌复合体（Bcc）菌株对秀丽隐杆线虫致病性的生物学试验。具体来说，这两种检测方法可以确定正在研究的Bcc菌株是否能够通过肠道定植（慢杀试验，SKA）或通过毒素产生（快杀试验，FKA）杀死线虫。这两种方法的主要区别在于对蠕虫的杀伤动力学不同。
This protocol describes two biological assays to evaluate pathogenicity of Burkholderia cepacia complex (Bcc) strains against the nematode Caenorhabditis elegans. Specifically, these two assays allow one to identify if the under-investigated Bcc strains are able to kill the nematodes by intestinal colonization (slow killing assay, SKA) or by toxins production (fast killing assay, FKA). The principal differences between the two assays rely on the different killing kinetics for worms.