Degradation of T-cell receptor chains in the endoplasmic reticulum is inhibited by inhibitors of cysteine proteases.
Degradation of T-cell receptor chains in the endoplasmic reticulum is inhibited by inhibitors of cysteine proteases.
复制标题
半胱氨酸蛋白酶抑制剂可抑制内质网中 T 细胞受体链的降解。
DOI：
10.1091/mbc.2.9.753
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
C. Terhorst
中科院分区：
文献类型：
--
作者：
T. Wileman;L. Kane;C. Terhorst
内质网或与之密切相关的细胞器含有蛋白酶，可用于去除部分组装或不正确折叠的蛋白质。目前对降解这些蛋白质的蛋白酶类型知之甚少。人T细胞抗原受体（TCR）的β链和分化簇（CD）3 δ亚基在合成后不久降解。在这项研究中，中国仓鼠卵巢（CHO）细胞转染β或δ与一组蛋白酶抑制剂孵育，并随后使用链特异性酶联免疫吸附试验（ELISA）的降解率的转染蛋白质。在测试的蛋白酶抑制剂中，两条链的降解对巯基试剂和半胱氨酸蛋白酶的肽基抑制剂高度敏感。几乎完全抑制内质网降解的抑制剂浓度不会引起细胞ATP水平的总体变化，也不会显著减缓CHO细胞的组成性分泌。抑制剂不影响CHO细胞合成和组装二硫键连接的TCR ζ二聚体的能力。我们的结论是，蛋白酶抑制剂是没有毒性的细胞，并没有影响内质网的生物合成活性。此外，它们没有改变内质网将其内容物输送到高尔基体的能力。总之，这些结果表明半胱氨酸蛋白酶抑制剂通过对半胱氨酸蛋白酶的作用减缓内质网中的降解。结果表明，内质网含有半胱氨酸蛋白酶，可用于去除保留的蛋白质。
The endoplasmic reticulum, or an organelle closely associated with it, contains proteases that can be used to remove partially assembled or improperly folded proteins. Very little is known at present about the types of protease that degrade these proteins. The beta chain and cluster of differentiation (CD)3 delta subunit of the human T-cell antigen receptor (TCR) are degraded shortly after synthesis. In this study Chinese hamster ovary (CHO) cells transfected with either beta or delta were incubated with a panel of protease inhibitors, and the rates of degradation of the transfected proteins were followed using chain-specific enzyme-linked immunosorbent assays (ELISAs). Of the protease inhibitors tested, degradation of both chains was highly sensitive to sulfhydryl reagents and peptidyl inhibitors of cysteine proteases. Concentrations of inhibitors that produced near complete inhibition of degradation in the endoplasmic reticulum did not cause gross changes in cellular ATP levels nor did they significantly slow constitutive secretion from CHO cells. The inhibitors did not affect the ability of CHO cells to synthesize and assemble disulphide-linked TCR zeta dimers. We conclude that the protease inhibitors were not toxic to cells and did not affect the biosynthetic activity of the endoplasmic reticulum. Furthermore, they did not alter the ability of the endoplasmic reticulum to deliver its content to the Golgi apparatus. Taken together, these results suggest that the cysteine protease inhibitors slow degradation in the endoplasmic reticulum through an action on cysteine proteases. The results imply that the endoplasmic reticulum contains cysteine proteases that can be used to remove retained proteins.
登录
查看更多内容
血清素结合蛋白：合成、分泌和回收。
影响因子：
4.7
作者：
Tamir,H;Liu,KP;Hsiung,S;Adlersberg,M;Gershon,MD
通讯作者：
Gershon,MD
T 细胞抗原受体 β 链的跨膜锚包含高尔基体前蛋白水解的结构决定因素。
DOI：
10.1091/mbc.1.12.907
发表时间：
1990
期刊：
Cell regulation
影响因子：
--
作者：
Wileman,T;Carson,GR;Shih,FF;Concino,MF;Terhorst,C
通讯作者：
Terhorst,C
细胞钙的消耗加速了内质网中的蛋白质降解。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wileman,T;Kane,LP;Carson,GR;Terhorst,C
通讯作者：
Terhorst,C
DOI：
--
发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rotundo,RL;Thomas,K;Porter-Jordan,K;Benson,RJ;Fernandez-Valle,C;Fine,RE
通讯作者：
Fine,RE