Metabolism of ubiquitinated histones.
Metabolism of ubiquitinated histones.
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泛素化组蛋白的代谢。
DOI：
10.1016/s0021-9258(19)69296-3
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
W. Bonner
中科院分区：
文献类型：
--
作者：
R. S. Wu;K. Kohn;W. Bonner
在动物染色质中，组蛋白2A's和2B's的一部分通过异肽连接共价连接到泛素蛋白上。在分裂细胞（如L1210和中国仓鼠卵巢细胞）和非分裂细胞（如未受刺激的淋巴细胞）中，发现H2A和H2B的泛素部分与游离泛素池快速平衡。泛素的合成和泛素化组蛋白的形成与DNA合成无关。所有泛素化组蛋白，包括四种H2A变体和H2B，在分离的中期染色体中都不存在。
In animal chromatin, a fraction of the histone 2A's and 2B's is covalently attached to the protein ubiquitin through an isopeptide linkage. The ubiquitin moieties of the H2A's and H2B's are found to be in rapid equilibrium with the pool of free ubiquitin, both in dividing cells such as L1210 and Chinese hamster ovary cells and in nondividing cells such as unstimulated lymphocytes. The synthesis of ubiquitin and the formation of ubiquitinated histones are not linked to DNA synthesis. All ubiquitinated histones, including the four H2A variants and the H2B's are absent from isolated metaphase chromosomes.