Low levels of faecal lactobacilli in women with iron-deficiency anaemia in south India
Low levels of faecal lactobacilli in women with iron-deficiency anaemia in south India
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DOI：
10.1017/s0007114510001637
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发表时间：
2010-10-01
影响因子：
3.6
通讯作者：
Ramakrishna, Balakrishnan S.
中科院分区：
文献类型：
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作者：
Balamurugan, Ramadass;Mary, R. Regina;Ramakrishna, Balakrishnan S.
在印度，妇女缺铁是造成母婴发病率的重要原因。肠道细菌群可促进盲肠和近端结肠对铁的吸收。本研究调查了贫血年轻女性的肠道微生物区系可能与Hb水平正常的女性不同。用实时荧光定量聚合酶链式反应检测了8名18-25岁贫血妇女(Hb=120g/L)粪便中的微生物区系。扩增了双歧杆菌属、嗜酸乳杆菌属、类杆菌-普氏杆菌-卟啉单胞菌属、平胸梭菌属和直肠真细菌的16S rDNA序列，并与普遍保守的细菌16S rDNA序列进行了相对差异表达。通过维持一周的饮食日记来确定能量、碳水化合物、纤维和铁的饮食摄入量。贫血妇女粪便乳酸杆菌数(中位数6.6x10(-8)，与细菌总数的相对差值)显著低于对照组(2.9x10(-6)；P=0.001，对数变换非配对t检验)。在接受检查的任何其他细菌方面，两组之间没有差异。两组的能量、碳水化合物、纤维、铁和牛奶的摄入量相似。印度南部年轻女性缺铁与粪便中乳酸菌含量低有关。乳酸菌与铁缺乏的关系有待进一步探讨。
Fe deficiency in women contributes significantly to maternal and child morbidity in India. The intestinal bacterial flora may facilitate absorption of Fe from the caecum and proximal colon. The present study investigated the possibility that intestinal microbiota of anaemic young women may differ from that of women with normal Hb levels. The microbiota was quantified by real-time PCR in faeces of eight anaemic (Hb = 120 g/l) women aged 18-25 years. Sequences of 16S ribosomal DNA (rDNA) specific to Bifidobacterium genus, Lactobacillus acidophilus group, Bacteroides-Prevotella-Porphyromonas group, Clostridium leptum group and Eubacterium rectale were amplified and expressed (as relative difference) relative to the universally conserved bacterial 16S rDNA sequences. Dietary intakes of energy, carbohydrate, fibre and Fe were ascertained by maintenance of a diet diary for a week. Faecal lactobacilli were significantly lower in anaemic women (median 6.6 x 10(-8), relative difference compared with total bacteria) than in the reference group (2.9 x 10(-6); P=0.001, unpaired t test with logarithmic transformation). There was no difference between the two groups with respect to any of the other bacteria that were examined. Intakes of energy, carbohydrate, fibre, Fe and milk were similar in both the groups. Fe deficiency in young women in south India was associated with low levels of lactobacilli in the faeces. The relationship between lactobacilli and Fe deficiency needs to be explored further.