Chlamydia trachomatis heat shock protein-60 induced interferon-γ and interleukin-10 production in infertile women
Chlamydia trachomatis heat shock protein-60 induced interferon-γ and interleukin-10 production in infertile women
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DOI：
10.1046/j.1365-2249.2003.02048.x
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发表时间：
2003-02-01
影响因子：
4.6
通讯作者：
Paavonen, J
中科院分区：
文献类型：
--
作者：
Kinnunen, A;Surcel, HM;Paavonen, J
沙眼衣原体相关输卵管因子不孕（TFI）涉及对衣原体60kda热休克蛋白（CHSP60）的体液和细胞介导的免疫反应增强。我们通过研究57例TFI妇女和76例其他原因不孕妇女的淋巴细胞增殖和细胞因子(干扰素（IFN)- γ、白细胞介素(IL)-12和IL-10）分泌对沙眼原体（EB）和CHSP60抗原的反应来评估CHSP60诱导的免疫应答在TFI中的作用。PBMC对CHSP60的阳性增殖反应在TFI组（20/57;36%）比其他组（17/76;22%）更为常见，尽管频率或中位反应没有显著差异（1.6，范围0.2-22.1 vs 1.4; 0.2-24.4）。与其他各组相比，TFI组沙眼衣原体EB诱导ifn - γ显著升高，IL-10分泌显著降低。在所有研究组中，EB和CHSP60诱导的IL-12分泌相似，与ifn - γ分泌相关，但在TFI组中不相关。eb诱导的IL-12和ifn - γ产生之间缺乏相关性，同时在TFI组中发现CHSP60反应中IL-10分泌显著，这表明CHSP60可能在衣原体感染期间调节免疫反应中具有特定作用，因此可能参与TFI的免疫发病机制。
Chlamydia trachomatis -associated tubal factor infertility (TFI) involves enhanced humoral and cell-mediated immune response to the chlamydial 60 kDa heat shock protein (CHSP60). We evaluated the role of CHSP60-induced immune response in TFI by studying lymphocyte proliferation and cytokine (interferon (IFN)-gamma , interleukin (IL)-12 and IL-10) secretion in response to C. trachomatis elementary body (EB) and CHSP60 antigens in 57 women with TFI and in 76 women with other causes of infertility. Positive proliferative response of PBMC to CHSP60 was more common in the TFI group (20/57; 36%) than in the other groups (17/76; 22%) although the frequency or the median responses did not differ significantly (1.6, range 0.2-22.1 versus 1.4; 0.2-24.4). C. trachomatis EB induced significantly higher IFN-gamma and lower IL-10 secretion in the TFI group compared to the other groups. The EB and CHSP60 induced IL-12 secretion was similar in all study groups and correlated with IFN-gamma secretion in the other but not in the TFI group. The lack of correlation between EB-induced IL-12 and IFN-gamma production and simultaneously found prominent IL-10 secretion in response to CHSP60 in the TFI group suggests that the CHSP60 may have a specific role in regulating the immune reactions during chlamydial infection and may consequently contribute to the immunopathogenesis of TFI.