Using FRET-Based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity

Using FRET-Based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity
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DOI:
10.1007/978-1-61779-160-4_16
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发表时间:
2011-01-01
期刊:
SIGNAL TRANSDUCTION PROTOCOLS, THIRD EDITION
影响因子:
--
通讯作者:
Zhang, Jin
Zhang, Jin
中科院分区:
其他
文献类型:
--
作者:
Depry, Charlene;Zhang, Jin

文献摘要

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The ubiquitous Protein Kinase A (PKA) signaling pathway is responsible for the regulation of numerous processes including gene expression, metabolism, cell growth, and cell proliferation. This method details how to monitor real-time PKA activity dynamics in mammalian cells using fluorescence resonance energy transfer (FRET)-based reporters.