A study of the genetic diversity in the Heze Han population using a novel genotyping system based on 24 Y-chromosomal STR loci
A study of the genetic diversity in the Heze Han population using a novel genotyping system based on 24 Y-chromosomal STR loci
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利用基于 24 个 Y 染色体 STR 位点的新型基因分型系统研究菏泽汉族人群遗传多样性
DOI：
10.1016/j.fsigss.2017.09.047
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发表时间：
2017-12-01
期刊：
影响因子：
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通讯作者：
Li, Chengtao
中科院分区：
文献类型：
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作者：
Li, Min;Que, Tingzhi;Li, Chengtao
我们建立了一个 24 重 Y-STR 分型系统，其中包括 AmpFLSTR (R) Yfiler (R) PCR 扩增试剂盒（Applied Biosystems，Foster City，CA）中使用的 13 个 Y-STR 位点和 11 个额外选择的 Y-STR 位点，这些位点显着提高了区分能力。我们将该系统应用于 195 个不相关的中国汉族男性的基因分型，产生了 195 个不同的单倍型。跨位点观察到的多样性范围为 0.0885 (DYS645) 至 0.9646 (DYS385a/b)。，而基于 24 个 Y-STR 位点的单倍型多样性为 1。基因分型系统的超高区分能力在法医学应用中具有巨大潜力。
We established a 24-plex Y-STR typing system by including the 13 Y-STR loci used in AmpFLSTR (R) Yfiler (R) PCR Amplification Kit (Applied Biosystems, Foster City, CA) and 11 additionally selected Y-STR loci that significantly increased power of discrimiation. We applied the system to genotype 195 unrelated Chinese Han males, resulting in 195 distinct haplotypes. The diversity observed across loci ranged from 0.0885 (DYS645) to 0.9646 (DYS385a/b)., while the haplotype diversity was 1 based on the 24 Y-STR loci. The ultra-high differentiation power of the genotyping system has great potential in forensic applications.