Quantitative imaging of nitrogen fixation by individual bacteria within animal cells
Quantitative imaging of nitrogen fixation by individual bacteria within animal cells
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DOI：
10.1126/science.1145557
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发表时间：
2007-09-14
期刊：
影响因子：
56.9
通讯作者：
Distel, Daniel L.
中科院分区：
文献类型：
--
作者：
Lechene, Claude P.;Luyten, Yvette;Distel, Daniel L.
生物固氮，将大气中的氮转化为氨用于生物合成，仅由少数细菌和古菌进行。尽管生物固氮的重要性，它一直是不可能量化的氮掺入个别细菌或映射的命运固定的氮在宿主细胞。在这项研究中，多同位素成像质谱法，我们直接成像和测量的氮固定的真核宿主细胞内的单个细菌，并证明了固定的氮用于宿主代谢。这种方法为研究微生物和全球营养循环提供了一种强有力的方法。
Biological nitrogen fixation, the conversion of atmospheric nitrogen to ammonia for biosynthesis, is exclusively performed by a few bacteria and archaea. Despite the essential importance of biological nitrogen fixation, it has been impossible to quantify the incorporation of nitrogen by individual bacteria or to map the fate of fixed nitrogen in host cells. In this study, with multi-isotope imaging mass spectrometry we directly imaged and measured nitrogen fixation by individual bacteria within eukaryotic host cells and demonstrated that fixed nitrogen is used for host metabolism. This approach introduces a powerful way to study microbes and global nutrient cycles.