Macrophage inhibitory cytokine 1 biomarker serum immunoassay in combination with PSA is a more specific diagnostic tool for detection of prostate cancer.
Macrophage inhibitory cytokine 1 biomarker serum immunoassay in combination with PSA is a more specific diagnostic tool for detection of prostate cancer.
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DOI：
10.1371/journal.pone.0122249
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Mandecki W
中科院分区：
文献类型：
--
作者：
Li J;Veltri RW;Yuan Z;Christudass CS;Mandecki W
前列腺癌（PCa）是美国男性中最常见的恶性肿瘤。尽管前列腺特异性抗原（PSA）检测具有高度敏感性，但其特异性较低，导致PCa的过度诊断和过度治疗。本文介绍了一项回顾性研究的结果，该研究表明，与PSA检测一起检测巨噬细胞抑制性细胞因子1（MIC-1）浓度沿着可大大提高检测的特异性。MIC-1血清水平通过一种新的基于p-Chip的免疫测定法在70个回顾性样本上进行测定。该测定被配置在p芯片上，p芯片是能够在其电子存储器中存储序列号以识别固定在其表面上的分子探针的小型集成电路（IC）。MIC-1和预定PSA浓度的分布显示在2D图中，并分析了MIC-1/PSA双重测定的预测能力。PCa患者血清中MIC-1浓度（1.44 ng/ml）高于正常和活检阴性个体（分别为0.93 ng/ml和0.88 ng/ml）。此外，MIC-1水平与PCa的进展相关。接受者操作曲线下面积（AUC-ROC）为0.81，通过使用MIC-1和PSA的逻辑回归值0.494的截止值，提供了83.3%的检测灵敏度和60.7%的特异性。另一种方法，通过在二维图中定义高频PCa区，导致检测灵敏度为78.6%，特异性为89.3%。基于血清中MIC-1和PSA浓度与患者PCa状态的相关性的分析提高了PCa诊断的特异性，而不影响单独PSA测试的高灵敏度，并且具有用于患者治疗策略的PCa预后的潜力。
Prostate cancer (PCa) is the most common malignancy among men in the United States. Though highly sensitive, the often-used prostate-specific antigen (PSA) test has low specificity which leads to overdiagnosis and overtreatment of PCa. This paper presents results of a retrospective study that indicates that testing for macrophage inhibitory cytokine 1 (MIC-1) concentration along with the PSA assay could provide much improved specificity to the assay. The MIC-1 serum level was determined by a novel p-Chip-based immunoassay run on 70 retrospective samples. The assay was configured on p-Chips, small integrated circuits (IC) capable of storing in their electronic memories a serial number to identify the molecular probe immobilized on its surface. The distribution of MIC-1 and pre-determined PSA concentrations were displayed in a 2D plot and the predictive power of the dual MIC-1/PSA assay was analyzed. MIC-1 concentration in serum was elevated in PCa patients (1.44 ng/ml) compared to normal and biopsy-negative individuals (0.93 ng/ml and 0.88 ng/ml, respectively). In addition, the MIC-1 level was correlated with the progression of PCa. The area under the receiver operator curve (AUC-ROC) was 0.81 providing an assay sensitivity of 83.3% and specificity of 60.7% by using a cutoff of 0.494 for the logistic regression value of MIC-1 and PSA. Another approach, by defining high-frequency PCa zones in a two-dimensional plot, resulted in assay sensitivity of 78.6% and specificity of 89.3%. The analysis based on correlation of MIC-1 and PSA concentrations in serum with the patient PCa status improved the specificity of PCa diagnosis without compromising the high sensitivity of the PSA test alone and has potential for PCa prognosis for patient therapy strategies.
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影响因子：
3.8
作者：
Rasiah, KK;Kench, JG;Henshall, SM
通讯作者：
Henshall, SM
影响因子：
2.1
作者：
Miller, MC;O'Dowd, GJ;Veltri, RW
通讯作者：
Veltri, RW
影响因子：
6.6
作者：
Khan, MA;Partin, AW;Veltri, RW
通讯作者：
Veltri, RW
DOI：
10.1093/jnci/95.12.868
发表时间：
2003-06-18
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
Draisma, G;Boer, R;de Koning, HJ
通讯作者：
de Koning, HJ
影响因子：
1.9
作者：
DELONG, ER;DELONG, DM;CLARKEPEARSON, DI
通讯作者：
CLARKEPEARSON, DI