Cellular internalization of polypeptide-based nanoparticles: Effects of size, shape and surface morphology
Cellular internalization of polypeptide-based nanoparticles: Effects of size, shape and surface morphology
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多肽纳米颗粒的细胞内化：尺寸、形状和表面形态的影响
DOI：
10.1039/c8bm01163j
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发表时间：
2018
影响因子：
6.6
通讯作者：
Xinquan Jiang
中科院分区：
文献类型：
--
作者：
Jiaxiao Xue;Zhou Guan;Xingyu Zhu;Jiaping Lin;Chunhua Cai;Xiao Jin;Yongsheng Li;Zhaoyang Ye;Wenjie Zhang;Xinquan Jiang
纳米颗粒（NPs）可以被细胞吸收，然而，NPs的结构特征对其细胞内化的影响尚未得到很好的研究。在这项工作中，细胞内化性能的各种纳米粒子，包括棒与螺旋表面（螺旋棒），与条纹图案表面（条纹球），与光滑表面（光滑球）的球体相结合的实验和理论模拟进行了研究。本研究主要探讨了纳米粒的大小、形状和表面形态对其细胞内化行为的影响。这些纳米颗粒由异硫氰酸荧光素（FITC）标记的聚（γ-苄基-L-谷氨酸）-嵌段-聚（乙二醇）（PBLG（FITC）-b-PEG）嵌段共聚物和PBLG或聚苯乙烯（PS）均聚物的混合物自组装。结果发现，具有较小的尺寸，棒状形状，螺旋/条纹表面形态的纳米粒子表现出更高的细胞内化效率。纳米粒的内化效率的这种差异可以归因于它们的表面积和内化途径的差异。该研究不仅可以指导具有增强的细胞内化效率的纳米载体的设计，而且可以加深我们对具有相似结构的天然NPs的内化行为的理解（例如，病毒）。
Nanoparticles (NPs) can be taken up by cells; however, the effects of the structural characteristics of NPs on their cellular internalization have not been well explored. In this work, cellular internalization performances of various NPs including rods with helical surface (helical rods), spheres with stripe-pattern surface (striped spheres), and spheres with smooth surface (smooth spheres) were investigated by a combination of experiments and theoretical simulations. This study focuses on the effects of the size, shape, and surface morphology on their cellular internalization behaviors. These NPs were self-assembled from mixtures of fluorescein isothiocyanate (FITC)-labelled poly(γ-benzyl-L-glutamate)-block-poly(ethylene glycol) (PBLG(FITC)-b-PEG) block copolymers and PBLG or polystyrene (PS) homopolymers. It was found that the NPs possessing smaller size, rod-like shape, and helical/striped surface morphology exhibit higher cellular internalization efficiency. Such differences in the internalization efficiency for the NPs can be attributed to the differences in both their surface areas and internalization pathways. This study could not only guide the design of nanocarriers with enhanced cellular internalization efficiency, but also deepen our understanding of the internalization behavior of natural NPs with similar structures (e.g., virus).