Unbiased proteomic screen for binding proteins to modified lysines on histone H3.
Unbiased proteomic screen for binding proteins to modified lysines on histone H3.
复制标题
DOI：
10.1002/pmic.200800600
复制
发表时间：
2009-05
期刊：
影响因子：
3.4
通讯作者：
Zhao, Yingming
中科院分区：
文献类型：
--
作者：
Chan, Doug W.;Wang, Yi;Wu, Meng;Wong, Jiemin;Qin, Jun;Zhao, Yingming
我们报告了一种灵敏的肽下拉方法，结合LC-MS/MS的蛋白质鉴定和光谱计数的定性丰度测量，以鉴定与含有二甲基赖氨酸4（H3 K4 me 2），二甲基赖氨酸9（H3 K9 me 2）或乙酰赖氨酸9（H3 K9 ac）的组蛋白H3尾结合的蛋白质。我们的研究鉴定了86种与所检查的组蛋白H3尾肽相关的核蛋白，包括7种已知的直接结合剂和16种具有保守的PHD指、布罗莫结构域和WD 40结构域的推定的直接结合剂。我们的蛋白质组学筛选的可靠性得到了以下事实的支持，即超过三分之一的蛋白质先前被描述为直接或间接与组蛋白H3尾相关。据我们所知，本文的结果是对H3 K4 me 2、H3 K9 me 2和H3 K9 ac相关蛋白的最全面的分析，将为研究组蛋白编码效应蛋白机制的研究人员提供有用的资源。
We report a sensitive peptide pull-down approach in combination with protein identification by LC-MS/MS and qualitative abundance measurements by spectrum counting to identify proteins binding to histone H3 tail containing dimethyl lysine 4 (H3K4me2), dimethyl lysine 9 (H3K9me2), or acetyl lysine 9 (H3K9ac). Our study identified 86 nuclear proteins that associate with the histone H3 tail peptides examined, including seven known direct binders and 16 putative direct binders with conserved PHD finger, bromodomain, and WD40 domains. The reliability of our proteomic screen is supported by the fact that more than one-third of the proteins identified were previously described to associate with histone H3 tail directly or indirectly. To our knowledge, the results presented here are the most comprehensive analysis of H3K4me2, H3K9me2, and H3K9ac associated proteins and will provide a useful resource for researchers studying the mechanisms of histone code effector proteins.
登录
查看更多内容
影响因子：
5.3
作者：
Citterio, E;Van Den Boom, V;Vermeulen, W
通讯作者：
Vermeulen, W
影响因子：
7
作者：
Chu, FX;Nusinow, DA;Burlingame, AL
通讯作者：
Burlingame, AL
影响因子：
7
作者：
Manes, Nathan P.;Gustin, Jean K.;Heffron, Fred
通讯作者：
Heffron, Fred
影响因子：
7
作者：
Beck, Hans Christian;Nielsen, Eva C.;Jensen, Ole Norregaard
通讯作者：
Jensen, Ole Norregaard
影响因子：
--
作者：
Jung, SY;Malovannaya, A;Qin, J
通讯作者：
Qin, J