Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components
Differential roles of TLR2 and TLR4 in recognition of gram-negative and gram-positive bacterial cell wall components
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DOI：
10.1016/s1074-7613(00)80119-3
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发表时间：
1999-10-01
期刊：
影响因子：
32.4
通讯作者：
Akira, S
中科院分区：
文献类型：
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作者：
Takeuchi, O;Hoshino, K;Akira, S
Toll样受体(Toll-like Receptor，TLR)2和TLR4参与识别多种细菌细胞壁成分，如脂多糖(LPS)。为了研究TLR2在体内的作用，我们产生了TLR2缺陷小鼠。与TLR4缺陷小鼠的内毒素无反应相反，TLR2缺陷小鼠对内毒素的反应程度与野生型小鼠相同。缺乏TLR2的巨噬细胞对几种革兰氏阳性细菌细胞壁以及金黄色葡萄球菌肽聚糖反应迟钝。缺乏TLR4的巨噬细胞缺乏对革兰氏阳性脂磷壁酸的反应。这些结果表明，TLR2和TLR4在体内识别不同的细菌细胞壁成分，TLR2在革兰氏阳性细菌识别中起主要作用。
Toll-like receptor (TLR) 2 and TLR4 are implicated in the recognition of various bacterial cell wall components, such as lipopolysaccharide (LPS). To investigate in vivo roles of TLR2, we generated TLR2-deficient mice. In contrast to LPS unresponsiveness in TLR4-deficient mice, TLR2-deficient mice responded to LPS to the same extent as wild-type mice. TLR2-deficient macrophages were hyporesponsive to several Gram-positive bacterial cell walls as well as Staphylococcus aureus peptidoglycan. TLR4-deficient macrophages lacked the response to Gram-positive lipoteichoic acids. These results demonstrate that TLR2 and TLR4 recognize different bacterial cell wall components in vivo and TLR2 plays a major role in Gram-positive bacterial recognition.