Location of ubiquinone-10 (CoQ-10) in phospholipid vesicles.
Location of ubiquinone-10 (CoQ-10) in phospholipid vesicles.
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泛醌 10 (CoQ-10) 在磷脂囊泡中的位置。
DOI：
10.1016/0005-2736(85)90162-2
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
M. Moore
中科院分区：
文献类型：
--
作者：
L. Michaelis;M. Moore
采用超声辐照法制备蛋黄磷脂酰胆碱（PC）脂质体。至少 25 mol% 的辅酶 Q-10 (CoQ-10) 可以非化学计量掺入 PC 脂质体中。电子显微镜显示 CoQ-10 对膜结构没有明显影响。含有 CoQ-10 的超声 PC 脂质体的核磁共振谱显示出两个峰（3.82 和 3.98 ppm），这是由于 CoQ-10 甲氧基质子和一个“高场成分”（1.52 和 1.58 ppm）。这些峰的面积呈反比关系并受超声波照射时间的影响。短时间超声处理后，低场位置 (3.98/1.58 ppm) 受到青睐，长时间超声处理后，高场位置 (3.82/1.52 ppm) 受到青睐。没有观察到两个峰的逐渐移动。发现“临界”脂质体直径在 500 至 700 Å 之间。镧系元素在 CoQ-10 共振（“高场分量”和甲氧基）上引起的赝接触位移不会导致峰分裂，并且它们之间的差异保持恒定。结论是，CoQ-10 被纳入膜核心，超出 PC 酰基链的 C-2，具有两个双层曲率依赖的共振位置。
Egg yolk phosphatidylcholine (PC) liposomes were prepared by ultrasonic irradiation. At least 25 mol% of coenzyme Q-10 (CoQ-10) can be incorporated nonstoichiometrically into PC liposomes. Electron microscopy showed no visible influence of CoQ-10 on the membrane structure. Nuclear magnetic resonance spectra of sonicated PC liposomes containing CoQ-10 showed two peaks (3.82 and 3.98 ppm) due to CoQ-10 methoxyl protons and a ‘high-field component’ (1.52 and 1.58 ppm). The areas of these peaks were inversely related and influenced by the time of ultrasonic irradiation. After short sonication the low-field positions (3.98/1.58 ppm) are favoured, after long sonication the high-field positions (3.82/1.52 ppm). No gradual shift of the two peaks is observed. The ‘critical’ liposome diameter was found to be between 500 to 700 Å. Lanthanide induced pseudocontact shift on CoQ-10 resonances (‘high-field component’ and methoxyl) does not lead to a split of the peaks and the difference between them remains constant. It is concluded that CoQ-10 is incorporated into the membrane core, beyond C-2 of the PC acyl chains, with two bilayer curvature-dependent resonance positions.
关于泛醌在磷脂酰胆碱双层中的定位。
DOI：
10.1016/0005-2728(84)90040-9
发表时间：
1984
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Stidham,MA;McIntosh,TJ;Siedow,JN
通讯作者：
Siedow,JN
不同链长的泛醌在人工膜囊泡中的位置和迁移率。
影响因子：
2.9
作者：
Ulrich,EL;Girvin,ME;Cramer,WA;Markley,JL
通讯作者：
Markley,JL