ADENYLYL CYCLASE AMINO-ACID SEQUENCE - POSSIBLE CHANNEL-LIKE OR TRANSPORTER-LIKE STRUCTURE
ADENYLYL CYCLASE AMINO-ACID SEQUENCE - POSSIBLE CHANNEL-LIKE OR TRANSPORTER-LIKE STRUCTURE
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DOI：
10.1126/science.2472670
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发表时间：
1989-06-30
期刊：
影响因子：
56.9
通讯作者：
GILMAN, AG
中科院分区：
文献类型：
--
作者：
KRUPINSKI, J;COUSSEN, F;GILMAN, AG
从牛脑文库中分离出编码腺苷酸环化酶的互补DNA。1134个残基的大部分氨基酸序列可分为疏水和亲水两组交替的结构域。两个大疏水结构域中的每一个似乎都包含六个跨膜跨度。这两个大亲水结构域中的每一个都包含一个序列，该序列与几个胍基环化酶的单个细胞质结构域同源；这些序列可能代表核苷酸结合位点。观察到腺苷酸环化酶与各种质膜通道和转运体之间意想不到的地形相似性。这种结构的复杂性暗示了这种重要酶可能具有的未被认识到的功能。
Complementary DNA''s that encode an adenylyl cyclase were isolated from a bovine brain library. Most of the deduced amino acid sequence of 1134 residues is divisible into two alternating sets of hydrophobic and hydrophillic domains. Each of the two large hydrophobic domains appears to contain six transmembrane spans. Each of the two large hydrophilic domains contains a sequence that is homologous to a single cytoplasmic domain of several guanylyl cyclases; these sequences may represent nucleotide binding sites. An unexpected topographical resemblance between adenylyl cyclase and various plasma membrane channels and transporters was observed. This structural complexity suggests possible, unappreciated functions for this important enzyme.