Pentosidine correlates with nanomechanical properties of human jaw bone
Pentosidine correlates with nanomechanical properties of human jaw bone
复制标题
DOI：
10.1016/j.jmbbm.2019.06.002
复制
发表时间：
2019-10-01
影响因子：
3.9
通讯作者：
Hosokawa, Ryuji
中科院分区：
文献类型：
--
作者：
Kawamura, Michihiko;Masaki, Chihiro;Hosokawa, Ryuji
种植体与宿主骨在手术部位的初始紧密贴壁是牙种植体骨结合的关键因素。由于材料水平上异常的胶原交联导致骨力学性能下降，在颌骨中积累的晚期糖基化终末产物可能导致牙种植体在初始整合阶段的潜在失效。在这里，我们证明了降低的蠕变变形阻力和降低的颌骨尺寸恢复与骨基质中高水平的戊糖苷积累一致，这可能与血浆中的戊糖苷水平相关。外周血样本和手术部位的皮质骨样本来自计划在下颌骨中进行牙种植的患者。血浆中的戊糖苷水平进行了评估。随后，通过拉曼显微光谱和纳米压痕，分别定量的相对pentosidine水平和颌骨的机械性能。纳米压痕测试显示，随着骨基质中相对戊糖苷水平的增加，骨样品的蠕变变形抗力和随时间变化的尺寸恢复降低。在动态压痕测试过程中，在各种频率下的较高tan δ值也表明粘弹性与颌骨基质中戊糖苷的相对强度相关。我们发现血浆中的戊糖苷水平与骨基质之间呈正相关，这反过来又降低了颌骨在材料水平上的力学性能。增加蠕变和减少颌骨的尺寸恢复可能会减少牙种植体在初始整合阶段的机械联锁。鉴于血浆戊糖苷水平与骨的机械性能之间可能存在相关性，血浆戊糖苷水平的测量可以作为一个新的指标，以评估颌骨基质的质量在种植手术前。
Initial intimate apposition between implant fixtures and host bone at the surgical site is a critical factor for osseointegration of dental implants. The advanced glycation end products accumulated in the jaw bone could lead to potential failure of a dental implant during the initial integration stage, because of the inferior bone mechanical property associated with the abnormal collagen cross-linking at the material level. Here, we demonstrate the lowered creep deformation resistance and reduced dimensional recovery of jaw bone in line with high levels of pentosidine accumulation in the bone matrix which likely correlate with the pentosidine level in blood plasma. Peripheral blood samples and cortical bone samples at the surgical site were obtained from patients scheduled for dental implants in the mandible. The pentosidine levels in blood plasma were assessed. Subsequently, the relative pentosidine levels and the mechanical properties of the jaw bone were quantified by Raman microspectroscopy and nanoindentation, respectively. The nanoindentation tests revealed less creep deformation resistance and reduced time-dependent dimensional recovery of bone samples with the increase in the relative pentosidine level in the bone matrix. Higher tan delta values at the various frequencies during the dynamic indentation tests also suggested that viscoelasticity is associated with the relative intensity of pentosidine in the jaw bone matrix. We found a positive correlation between the pentosidine levels in blood plasma and the bone matrix, which in turn reduced the mechanical property of the jaw bone at the material level. Increased creep and reduced dimensional recovery of the jaw bone may diminish the mechanical interlocking of dental implants during the initial integration stage. Given the likely correlation between the plasma pentosidine level and the mechanical properties of bone, measurement of the plasma pentosidine level could serve as a new index to assess jaw bone matrix quality in advance of implant surgery.