Hybrid lethality caused by two complementary dominant genes in cabbage (Brassica oleracea L.)
Hybrid lethality caused by two complementary dominant genes in cabbage (Brassica oleracea L.)
复制标题
卷心菜 (Brassica oleracea L.) 中两个互补显性基因引起的混合致死率
DOI：
10.1007/s11032-016-0498-3
复制
发表时间：
2016-06-01
影响因子：
3.1
通讯作者：
Zhuang, Mu
中科院分区：
文献类型：
--
作者：
Hu, Yang;Xue, Yu-qian;Zhuang, Mu
杂种致死性是一种合子后生殖障碍，对物种具有重要意义。发现新的杂交致死病例并分析相应的致病基因可能为生殖隔离的建立和维持提供新的见解。本研究对甘蓝自交系09-211和09-222的杂种致死现象进行了观察。遗传分析表明，杂种致死力分别受09-211和09-222的两对互补显性基因BolC.HL1.a和BolC.HL2.a控制。进一步的分析表明，这两个基因符合Bateson-Dobzhansky-Muller模型。杂交致死基因的精细定位结果表明，BolC.HL1.a通过插入缺失HL 132和HL 134定位在C 01染色体上，遗传距离分别为0.2和0.1cM。两个标记之间的间隔距离为101 kb。BolC.HL2.a通过HL 235和HL 234分别以0.3和0.3cM的距离精细定位在C 04染色体上。物理距离为70 kb。这些研究结果为进一步克隆甘蓝杂种致死基因奠定了基础，有助于我们对甘蓝杂种致死的分子和进化机制的理解。
Hybrid lethality, a type of postzygotic reproductive barrier, is important for species. Discovering novel hybrid lethality cases and analyzing corresponding causal genes may provide new insights into the establishment and maintenance of reproductive isolation. In this study, we observed the hybrid lethality phenomena in a cross between two cabbage inbred lines, 09-211 and 09-222. Genetic analysis revealed that the hybrid lethality was controlled by two complementary dominant genes, BolC.HL1.a and BolC.HL2.a, from 09-211 and 09-222, respectively. Further analysis indicated that the two genes conform to the Bateson-Dobzhansky-Muller model. Fine mapping of hybrid lethal genes revealed that BolC.HL1.a was located on the C01 chromosome by Indels HL132 and HL134, with a genetic distance of 0.2 and 0.1 cM, respectively. The interval distance between the two markers was 101 kb. BolC.HL2.a was fine-mapped on the C04 chromosome by HL235 and HL234 at a distance of 0.3 and 0.3 cM, respectively. The physical distance was 70 kb. These findings lay the foundation for cloning the hybrid lethality genes in the future and contribute to our understanding of the molecular and evolutionary mechanisms of hybrid lethality in Brassica oleracea.