Analysis of Nanomaterial Toxicity by Western Blot.
Analysis of Nanomaterial Toxicity by Western Blot.
复制标题
通过蛋白质印迹分析纳米材料毒性。
DOI：
10.1007/978-1-4939-8916-4_10
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Jiang,Mizu
中科院分区：
文献类型：
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作者：
Long,Gao;Mo,Yiqun;Zhang,Qunwei;Jiang,Mizu
蛋白质印迹是一种常规生化技术，用于免疫检测暴露于纳米材料 (NM) 的细胞和组织中的蛋白质。它是一种灵敏的蛋白质分析方法，涉及通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 (SDS-PAGE) 溶解和分离蛋白质，将蛋白质转移并固定到固体支持物上，以及对特定抗原进行靶向免疫探测。蛋白质印迹作为一种方便、可靠的研究工具，与质谱、蛋白质芯片一起在蛋白质组学时代发挥着不可替代的作用。在本章中，我们描述了从样品制备到目标蛋白定量测量的整个过程的详细方案。
Western blot is a routine biochemical technique for the immunodetection of proteins in cells and tissues exposed to nanomaterials (NMs). It is a sensitive method for protein analysis that involves the solubilization and separation of proteins by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), transferring and immobilizing proteins onto a solid support, and targeted immunoprobing of a specific antigen. As a convenient and reliable research tool, the western blot plays an irreplaceable role in the era of proteomics together with mass spectrometry and protein chip. In this chapter we describe the detailed protocol for the entire process from sample preparation to quantitative measurement of target proteins.