Electrochemical studies of the interaction of the anticancer herbal drug emodin with DNA.
Electrochemical studies of the interaction of the anticancer herbal drug emodin with DNA.
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DOI：
10.1016/j.jpba.2006.05.017
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发表时间：
2006-11
影响因子：
3.4
通讯作者：
Liping Wang;Lin Lin-Lin;B. Ye
中科院分区：
文献类型：
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作者：
Liping Wang;Lin Lin-Lin;B. Ye
采用差示脉冲伏安法（DPV）和循环伏安法（CV）研究了抗癌中药大黄素与DNA的相互作用。DPV结果表明，DNA的加入使大黄素的峰电位正移，峰电流减小。紫外光谱显示大黄素在440 nm处的吸收峰随波长的红移而减弱。结果表明，大黄素与DNA的相互作用是通过插入DNA的双螺旋结构进行的。在本实验条件下，峰电流的降低与DNA浓度成正比，可用于DNA浓度的测定。测定了DNA-mEM的结合常数（β）和结合数（m）。
The interaction between anticancer herbal drug emodin and DNA was studied with differential pulse voltammetry (DPV) and cyclic voltammetry (CV) at the bare or DNA modified GCE and ultraviolet–visible (UV) spectra. The DPV of emodin showed that peak potentials shifted to more positive value and peak currents decreased with the addition of DNA. UV spectra exhibited that the absorption of emodin at about 440nm decreased with red shift. The results showed that the herbal drug emodin interacted with DNA by intercalating into the double helix of DNA. Under our experiment conditions, the decrease of peak current was proportional to DNA concentration, which can be applied to determining DNA concentration. The combining constant (β) and combing number (m) of DNA–mEM were determined too.