Morphological Response in Cancer Spheroids for Screening Photodynamic Therapy Parameters.
Morphological Response in Cancer Spheroids for Screening Photodynamic Therapy Parameters.
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DOI：
10.3389/fmolb.2021.784962
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发表时间：
2021
影响因子：
5
通讯作者：
Claeyssens F
中科院分区：
文献类型：
--
作者：
Aguilar Cosme JR;Gagui DC;Bryant HE;Claeyssens F
光动力疗法（PDT）是一种利用光激活化合物产生活性氧，导致膜损伤和细胞死亡的治疗方法。与单层细胞培养物相比，多细胞癌球体是PDT评价的优选替代物，因为它们能够更好地模拟体内无血管肿瘤特征，例如缺氧和细胞-细胞相互作用，成本低，易于生产。然而，不一致的生长动力学和药物反应性导致实验重现性差，并限制了它们的有用性。在此，我们使用图像分析来建立人类黑色素瘤C8161球体形态和药物反应性之间的联系。基于球度、面积和直径预先选择球体，减少处理前实验组中的变化。使用AnaSP和ReViSP（基于MATLAB的开源软件）分析PDT后的球体形态，获得9个不同的参数。球状体在生物测定和形态学之间显示出线性响应，面积（R2 = 0.7219）和体积（R2 = 0.6138）显示出最佳拟合。球形度、凸度和实度被确认为球体活力的较差独立指标。我们的研究结果表明，球体形态参数可以用来准确筛选无效的治疗组合的新化合物。
Photodynamic therapy (PDT) is a treatment which uses light-activated compounds to produce reactive oxygen species, leading to membrane damage and cell death. Multicellular cancer spheroids are a preferable alternative for PDT evaluation in comparison to monolayer cell cultures due to their ability to better mimic in vivo avascular tumour characteristics such as hypoxia and cell-cell interactions, low cost, and ease of production. However, inconsistent growth kinetics and drug responsiveness causes poor experimental reproducibility and limits their usefulness. Herein, we used image analysis to establish a link between human melanoma C8161 spheroid morphology and drug responsiveness. Spheroids were pre-selected based on sphericity, area, and diameter, reducing variation in experimental groups before treatment. Spheroid morphology after PDT was analyzed using AnaSP and ReViSP, MATLAB-based open-source software, obtaining nine different parameters. Spheroids displayed a linear response between biological assays and morphology, with area (R2 = 0.7219) and volume (R2 = 0.6138) showing the best fit. Sphericity, convexity, and solidity were confirmed as poor standalone indicators of spheroid viability. Our results indicate spheroid morphometric parameters can be used to accurately screen inefficient treatment combinations of novel compounds.
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DOI：
10.3791/51639
发表时间：
2014-07-08
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Chen W;Wong C;Vosburgh E;Levine AJ;Foran DJ;Xu EY
通讯作者：
Xu EY
影响因子：
3.7
作者：
Ivanov DP;Parker TL;Walker DA;Alexander C;Ashford MB;Gellert PR;Garnett MC
通讯作者：
Garnett MC
ECM 成分和流变学调节胰腺导管腺癌 3D 模型中的生长、运动和对光动力治疗的反应。
DOI：
10.1158/1541-7786.mcr-16-0260
发表时间：
2017-01
期刊：
Molecular cancer research : MCR
影响因子：
--
作者：
Cramer GM;Jones DP;El-Hamidi H;Celli JP
通讯作者：
Celli JP
DOI：
10.1016/j.jconrel.2012.04.045
发表时间：
2012-12-10
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Mehta G;Hsiao AY;Ingram M;Luker GD;Takayama S
通讯作者：
Takayama S
影响因子：
3.5
作者：
Anbil, Sriram;Rizvi, Imran;Hasan, Tayyaba
通讯作者：
Hasan, Tayyaba