Transcriptomic and metabolomic analyses reveal antibacterial mechanism of astringent persimmon tannin against Methicillin-resistant Staphylococcus aureus isolated from pork
Transcriptomic and metabolomic analyses reveal antibacterial mechanism of astringent persimmon tannin against Methicillin-resistant Staphylococcus aureus isolated from pork
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DOI：
10.1016/j.foodchem.2019.125692
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发表时间：
2020-03-20
期刊：
影响因子：
8.8
通讯作者：
Fan, Mingtao
中科院分区：
文献类型：
--
作者：
Liu, Miaomiao;Feng, Mingxing;Fan, Mingtao
紫苏单宁（PT）对从零售猪肉中分离的耐甲氧西林金黄色葡萄球菌（MRSA）具有抗菌活性。本研究首次采用转录组和代谢组的方法研究了其分子机制。结果表明，PT的亚抑制浓度（0.5 mg/ml）诱导MRSA在转录和代谢水平的显着变化，370个基因和19个代谢产物的差异表达。生物信息学分析表明，变异基因和代谢产物主要涉及膜转运、氨基酸、碳水化合物和能量代谢途径。其中渗透调节、细胞内pH调节、氨基酸合成与代谢、糖酵解、TCA循环和铁代谢的变化最为突出，提示PT对细胞膜损伤、氨基酸限制、能量代谢紊乱和铁剥夺等多方面的影响。研究结果为PT的抗MRSA作用机制提供了新的认识，为PT在食品安全领域的开发和应用提供了理论依据。
Persimmon tannin (PT) exhibits antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) isolated from retail pork. The involved molecular mechanisms were investigated for the first time using transcriptome and metabolome in this study. Results showed that subinhibitory concentration of PT (0.5 mg/ml) induced significant changes in MRSA at both transcriptional and metabolic levels, as 370 genes and 19 metabolites were differentially expressed. Bioinformatic analysis revealed that the varying genes and metabolites were mainly involved in pathways of membrane transport, amino acids, carbohydrate, and energy metabolism. The highlighted changes were those related to osmotic regulation, intracellular pH regulation, amino acid synthesis and metabolism, glycolysis, TCA cycle and iron metabolism, suggesting the multifaceted effects including cell membrane damage, amino acids limitation, energy metabolism disorder and iron deprivation induced by PT. The results provided insight into the anti-MRSA mechanism of PT, which is useful for PT's development and application in food safety.