GENETIC-TRANSFORMATION IN STREPTOCOCCUS-PNEUMONIAE - NUCLEOTIDE-SEQUENCE ANALYSIS SHOWS COMA, A GENE REQUIRED FOR COMPETENCE INDUCTION, TO BE A MEMBER OF THE BACTERIAL ATP-DEPENDENT TRANSPORT PROTEIN FAMILY
GENETIC-TRANSFORMATION IN STREPTOCOCCUS-PNEUMONIAE - NUCLEOTIDE-SEQUENCE ANALYSIS SHOWS COMA, A GENE REQUIRED FOR COMPETENCE INDUCTION, TO BE A MEMBER OF THE BACTERIAL ATP-DEPENDENT TRANSPORT PROTEIN FAMILY
复制标题
DOI：
10.1128/jb.173.1.372-381.1991
复制
发表时间：
1991-01-01
影响因子：
3.2
通讯作者：
MORRISON, DA
中科院分区：
文献类型：
--
作者：
HUI, FM;MORRISON, DA
利用质粒DNA模板和合成的寡核苷酸引物，测定了肺炎链球菌中诱导感受态转化所需基因comA的全序列。 该序列含有一个大的开放阅读框，ORF 1，为2,151 bp。 ORF 1被包括在comAB基因座先前映射的遗传和占其范围的50%。ORF 1中编码的最大多肽的预测分子量为80，290，与先前克隆的comA基因座的体外转录-翻译产物的测量分子量（77，000）一致。一个Shine-Dalgarno序列（AAAGGAG，Δ G = -14 kcal）位于ORF 1的上游。 与大肠杆菌sigma-70启动子共有序列相似的序列（TTtAat-17 bp-TAaAAT）位于ORF 1上游410 bp处。 推导的ComA蛋白序列与大肠杆菌的同源性很高。大肠杆菌溶血素分泌蛋白HlyB与ATP依赖性转运蛋白家族的其他成员（包括哺乳动物多药耐药P-糖蛋白）有很强的相似性。这些相似之处表明，ComA的功能，在运输的一些分子，可能是肺炎球菌的能力因子本身。
The complete nucleotide sequence of comA, a gene required for induction of competence for genetic transformation in Streptococcus pneumoniae, was determined by using plasmid DNA templates and synthetic oligonucleotide primers. The sequence contained a single large open reading frame, ORF1, of 2,151 bp. ORF1 was included within the comAB locus previously mapped genetically and accounted for 50% of its extent. The predicted molecular weight of the largest polypeptide encoded within ORF1, 80,290, coincided with that measured previously (77,000) for the product of in vitro transcription-translation of the cloned comA locus. A Shine-Dalgarno sequence (AAAGGAG, DELTA-G = -14 kcal) lay immediately upstream of ORF1. A sequence (TTtAat-17 bp-TAaAAT) similar to the Escherichia coli sigma-70 promoter consensus was located 410 bp upstream of ORF1. The deduced protein sequence of ComA showed a very strong similarity to the E. coli hemolysin secretion protein, HlyB, and strong similarities to other members of the family of ATP-dependent transport proteins, including the mammalian multidrug resistance P-glycoprotein. These similarities suggest that ComA functions in the transport of some molecule, possibly pneumococcal competence factor itself.