A Golgi-specific protein PAQR3 is closely associated with the progression, metastasis and prognosis of human gastric cancers
A Golgi-specific protein PAQR3 is closely associated with the progression, metastasis and prognosis of human gastric cancers
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高尔基体特异性蛋白PAQR3与人胃癌的进展、转移和预后密切相关
DOI：
10.1093/annonc/mdu168
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发表时间：
2014-07-01
影响因子：
50.5
通讯作者：
Chen, Y.
中科院分区：
文献类型：
--
作者：
Ling, Z. Q.;Guo, W.;Chen, Y.
本研究从mRNA和蛋白水平检测了300例胃癌及其癌旁正常组织（PCHNT）中PAQR 3的表达情况，探讨PAQR 3与胃癌的发生、转移和生存的关系。分析PAQR 3表达水平与转移和预后等临床特征的相关性。PAQR 3对胃癌细胞生长和迁移的影响也进行了研究，结果表明，PAQR 3在胃癌组织中的表达在mRNA和蛋白水平均显著低于PCHNT（P均<0. 0001）。PAQR 3表达水平与幽门螺杆菌感染（P < 0.0001）、肿瘤大小（P <0.0001）、肿瘤分期（P <0.0001）、静脉和淋巴结浸润（P <0.0001）、远处和淋巴结转移（P < 0.0001）及患者生存期（P < 0.0001）呈负相关。PAQR 3的下调与胃癌样本中上皮-间质转化（EMT）的增加高度相关。此外，PAQR 3的过表达能够负性调节胃癌细胞的增殖、迁移和EMT，PAQR 3在人胃癌中表达显著下调。PAQR 3表达水平与胃癌的进展和转移密切相关。PAQR 3也是一个新的基因标记，可以预测胃癌患者的预后。
The aim of this study is to determine whether PAQR3, a protein specifically localized in the Golgi apparatus, is associated with tumor progression, metastasis and survival of human patients with gastric cancer.PAQR3 expression status was investigated in a large panel of gastric cancer (n = 300) and their corresponding para-cancerous histological normal tissues (PCHNT) at both mRNA and protein levels. The correlation of PAQR3 expression levels with clinical features such as metastasis and prognosis was analyzed. The effect of PAQR3 on the growth and migration of gastric cancer cells was also determined.PAQR3 was frequently down-regulated in gastric cancer samples compared with PCHNT at both mRNA and protein levels (both P < 0.0001). The expression level of PAQR3 was negatively correlated with Helicobacter pylori infection (P < 0.0001), tumor size (P < 0.0001), tumor stage (P < 0.0001), venous and lymphatic invasion (P < 0.0001), distant and nodal metastasis (P < 0.0001), and patient survival (P < 0.0001). Down-regulation of PAQR3 was highly correlated with increased epithelial-mesenchymal transition (EMT) in gastric cancer samples. In addition, PAQR3 overexpression was able to negatively modulate cell proliferation, migration and EMT of gastric cancer cells.PAQR3 is markedly down-regulated in human gastric cancers. PAQR3 expression level is closely associated with the progression and metastasis of gastric cancers. PAQR3 is also a new genetic signature that can predict the prognosis of the patients with gastric cancer.