Radial dispersion of red blood cells in blood flowing through glass capillaries: The role of hematocrit and geometry
Radial dispersion of red blood cells in blood flowing through glass capillaries: The role of hematocrit and geometry
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DOI：
10.1016/j.jbiomech.2008.04.033
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发表时间：
2008-07-19
影响因子：
2.4
通讯作者：
Yamaguchi, Takami
中科院分区：
文献类型：
--
作者：
Lima, Rui;Ishikawa, Takuji;Yamaguchi, Takami
血液在微循环中的流动特性强烈地依赖于血细胞比容（Hct）。微血管几何形状。和细胞特性。先前的体外研究已经使用常规显微镜测量了浓缩悬浮液中红细胞（RBC）的径向位移。然而，为了测量RBS的运动，他们使用了透明的幽灵红细胞悬浮液。其可能具有与正常RBC不同的物理性质。本研究介绍了一种新的方法（共聚焦micro-PTV）来测量标记的红细胞在正常红细胞的浓缩悬浮液中的运动。共聚焦系统获得薄焦平面的能力允许US精确测量单个RBC的径向位置，从而测量多个标记RBC之间的相互作用。所有的测量都是在中心平面的50和100 μ m的玻璃毛细管在雷诺数（Re）前0.003至0.005使用Hcts前2%至35%。为了量化多个RBC的运动和相互作用，我们使用RBC径向离散度（D-yy）。我们的结果清楚地表明，D-yy强烈依赖于Het。红细胞在0.4 - 0.8R之间的径向位置处表现出较高的D-yy，而在邻近壁的位置（0.8-1 R）和毛细血管中部周围（0- 0.2R）处表现出较低的D-yy。本研究所提供的信息不仅补充了以前对稀释和浓缩红细胞悬液的微血液流变学的研究。而且还显示了Hct和几何形状对RBC径向分散的影响。这些信息对于更好地理解生理和病理条件下的血液质量运输机制非常重要。(C)2008爱思唯尔有限公司版权所有。
The flow properties of blood in the microcirculation depend strongly on the hematocrit (Hct). microvessel geometry. and cell properties. Previous ill vitro studies have measured the radial displacement of red blood cells (RBCs) at concentrated Suspensions using conventional microscopes. However, to measure the RBSs motion they used transparent suspensions of ghost red cells. which may have different physical properties than normal RBCs. The present Study introduces a new approach (confocal micro-PTV) to measure the motion of labeled RBCs flowing in concentrated Suspensions of normal RBCs. The ability of confocal systems to obtain thin in-focus planes allowed US to measure the radial position of individual RBCs accurately and to consequently measure the interaction between multiple labeled RBCs. All the measurements were performed in the center plane of both 50 and 100 mu m glass capillaries at Reynolds numbers (Re) front 0.003 to 0.005 using Hcts front 2% to 35%. To quantify the Motion and interaction of multiple RBCs, we used the RBC radial dispersion (D-yy). Our results clearly demonstrate that D-yy strongly depends on the Het. The RBCs exhibited higher D-yy at radial positions between 0.4 and 0.8 R and lower D-yy at locations adjacent to the wall (0.8-1 R) and around the middle of the capillary (0-0.2 R) The present work also demonstrates that D-yy tends to decrease with a decrease in the diameter. The information provided by this study not only complements previous investigations oil microhemorheology of both dilute and concentrated Suspensions of RBCs. but also shows the influence of both Hct and geometry on the radial dispersion of RBCs. This information is important for a better Understanding of blood mass transport mechanisms under both physiological and pathological conditions. (C) 2008 Elsevier Ltd. All rights reserved.