Generation and analysis of Elf5-LacZ mouse: unique and dynamic expression of Elf5 (ESE-2) in the inner root sheath of cycling hair follicles
Generation and analysis of Elf5-LacZ mouse: unique and dynamic expression of Elf5 (ESE-2) in the inner root sheath of cycling hair follicles
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DOI：
10.1007/s00418-007-0347-x
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发表时间：
2008-01-01
影响因子：
2.3
通讯作者：
Sinha, Satrajit
中科院分区：
文献类型：
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作者：
Choi, Yeon Sook;Cheng, Jun;Sinha, Satrajit
Elf 5/ESE-2转录因子是上皮特异性Ets转录因子亚家族的成员。已知Elf 5的表达限于富含腺或分泌上皮细胞的器官和组织，如肾脏和乳腺以及皮肤的分化角质形成细胞。我们设计了一种Elf 5-LacZ小鼠品系，其中Elf 5基因的大部分编码区已被β-半乳糖苷酶（LacZ）报告基因和新霉素抗性盒取代。我们在这里表明，LacZ基因在Elf 5-LacZ小鼠的表达发生在空间和时间模式，模拟内源性Elf 5表达的观察到的强X-Gal染色在乳腺腔上皮细胞的杂合子Elf 5-LacZ动物。我们的分析还揭示了以前未发现的表达位点的Elf 5在分化细胞的内根鞘的毛囊。产生一种新的Elf 5基因靶向小鼠模型窝藏LacZ报告基因提供了一个优势，在体内研究Elf 5由于高灵敏度和易于原位检测LacZ基因的表达，通过组织化学染色与X-Gal。总之，这项研究为Elf 5表达的新模式带来了新的见解，这些模式可能与毛囊周期中功能重要的控制相关。
The Elf5/ESE-2 transcription factor is a member of the Epithelium Specific Ets subfamily of Ets transcription factors. Expression of Elf5 has been known to be restricted to organs and tissues rich in glandular or secretory epithelial cells such as kidney and mammary gland as well as differentiated keratinocytes of the skin. We have engineered an Elf5-LacZ mouse strain in which the bulk of the coding region of the Elf5 gene has been replaced by the beta-galactosidase (LacZ) reporter gene and the neomycin resistance cassette. We show here that LacZ gene expression in Elf5-LacZ mice occurs in spatial and temporal patterns that mimic endogenous Elf5 expression as observed by strong X-Gal staining in the mammary luminal epithelial cells of heterozygous Elf5-LacZ animals. Our analysis also reveals previously undiscovered expression site for Elf5 in the differentiated cells of the inner root sheath of hair follicle. The generation of a novel Elf5 gene targeted mouse model harboring a LacZ reporter gene provides an advantage for in vivo studies of Elf5 due to the highly sensitive and easy in situ detection of LacZ gene expression through histochemical staining with X-Gal. Taken together, this study brings new insights into novel patterns of Elf5 expression likely correlating with functionally important control in hair follicle cycle.