Common marmoset CD117-positive hematopoietic cells possess multipotency
Common marmoset CD117-positive hematopoietic cells possess multipotency
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普通狨猴 CD117 阳性造血细胞具有多能性
DOI：
10.1093/intimm/dxv031
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Kametani Y.
中科院分区：
文献类型：
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作者：
Shimada S;Nunomura S;Mori S;Suemizu H;Itoh T;Takabayashi S;Okada Y;Yahata T;Shiina T;Katoh H;Suzuki R;Tani K;Ando K;Yagita H;Habu S;Sasaki E;Kametani Y.
对非人灵长类造血的研究对于阐明灵长类造血的进化和免疫调节具有重要意义。然而，灵长类造血系统的植入和发育仅在人类中有充分的记录，而在非人类灵长类中尚不清楚。猕猴（学名：Callithrix jacchus，普通绒猴，CM）是一种新大陆猴，具有高怀孕率和小体型，生活在封闭的群体中。由于干细胞因子（SCF）是小鼠和人类造血干细胞发育的必需分子，我们将重点放在SCF受体CD 117上，并检查表达CD 117的细胞是否具有新生绒猴来源的造血细胞的造血干/祖细胞特征，这些造血细胞可以发育为T细胞和B细胞。当骨髓的CD 117+细胞部分被移植到免疫缺陷NOD（非肥胖糖尿病）/Shi-scid、普通γ c-null（NOG）小鼠中时，这些细胞有效地移植到NOG小鼠的骨髓和脾脏中。CD 117+细胞在体内可分化为髓系细胞、CD 20 +B细胞和CD 3 +T细胞，并可表达CM细胞因子。B细胞的发育并不先于T细胞。NOG小鼠CD 8 +T细胞的发育占主导地位。CD 117 + CD 34+细胞和CD 117 + CD 34 −细胞的植入相当。这些结果表明，CD 117+细胞部分可以分化成所有三种细胞谱系，并在异种环境中的绒猴免疫的发展遵循不同的发展途径相比，人类免疫。
Analysis of the hematopoiesis of non-human primates is important to clarify the evolution of primate-specific hematopoiesis and immune regulation. However, the engraftment and development of the primate hematopoietic system are well-documented only in humans and are not clear in non-human primates.Callithrix jacchus(common marmoset, CM) is a New World monkey with a high rate of pregnancy and small size that lives in closed colonies. As stem cell factor (SCF) is an essential molecule for hematopoietic stem cell development in mice and humans, we focused on CD117, the SCF receptor, and examined whether CD117-expressing cells possess the hematopoietic stem/progenitor cell characteristics of newborn marmoset-derived hematopoietic cells that can develop into T cells and B cells. When CD117+cell fractions of the bone marrow were transplanted into immunodeficient NOD (non-obese diabetic)/Shi-scid, common γc-null (NOG) mice, these cells engrafted efficiently in the bone marrow and spleens of the NOG mice. The CD117+cells developed into myeloid lineage cells, CD20+B cells and CD3+T cells, which could express CM cytokinesin vivo. The development of B cells did not precede that of T cells. The development of CD8+T cells was dominant in NOG mice. The engraftment was comparable for both CD117+CD34+cells and CD117+CD34−cells. These results suggest that the CD117+cell fraction can differentiate into all three cell lineages, and the development of marmoset immunity in the xenogeneic environment follows diverse developmental pathways compared with human immunity.