Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity
Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity
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DOI：
10.4049/jimmunol.164.4.1862
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发表时间：
2000-02-15
影响因子：
4.4
通讯作者：
Lehmann, PV
中科院分区：
文献类型：
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作者：
Karulin, AY;Hesse, MD;Lehmann, PV
在正常的、体内启动的CD4记忆T细胞中，Ag诱导的细胞因子共表达的模式仍然存在争议，因为这些细胞出现的低频率有效地阻碍了直接的体外测量，我们通过使用双色细胞因子酶联免疫斑点分析和计算机辅助图像分析克服了这一限制。我们发现同时表达IL-2、IL-3、IL-4、IL-5和干扰素-γ的CD4记忆细胞很少见(0-10%)，这种细胞因子分离在佐剂诱导的1型、2型和对OVA的混合免疫中可见，在利什曼原虫感染中，无论使用的Ag剂量或免疫后多久，这种细胞因子分离都是明显的。这些数据表明，体内的1型和2型免疫不是由经典的Th1或Th2细胞介导的，而是由产生单一细胞因子的记忆细胞介导的。
The patterns of Ag-induced cytokine coexpression in normal, in vivo-primed CD4 memory T cells has remained controversial because the low frequency at which these cells occur has effectively prevented direct ex vivo measurements, We have overcome this limitation by using two-color cytokine enzyme-linked immunospot assays and computer-assisted image analysis. We found CD4 memory cells that simultaneously expressed IL-2, IL-3, IL-4, IL-5, and IFN-gamma to be rare (0-10%), This cytokine segregation was seen in adjuvant-induced type 1, type 2, and mixed immunity to OVA, in Leishmania infection regardless of the Ag dose used or how long after immunization the assay was performed. The data suggest that type 1 and type 2 immunity in vivo is not mediated by classic Th1 or Th2 cells but by single-cytokine-producing memory cells.