Kappa-selenocarrageenan-induced apoptosis of multidrug-resistant human leukemia cell and its mechanism
Kappa-selenocarrageenan-induced apoptosis of multidrug-resistant human leukemia cell and its mechanism
复制标题
DOI：
--
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Wei Hu-lai
中科院分区：
文献类型：
--
作者：
Wei Hu-lai
目的：观察硒化角叉菜胶（KCS）诱导K562/ADM细胞凋亡的作用，并探讨其可能的分子机制。方法：采用MTT法、瑞氏-姬姆萨染色、DNA琼脂糖凝胶电泳及细胞周期分析等方法检测K562/ADM细胞凋亡情况。流式细胞仪检测Fas、Bcl-2、P53蛋白表达。采用RP-PCR法检测caspase-3 mRNA的表达。结果：KCS可抑制K562/ADM细胞的增殖;光镜下观察细胞凋亡的典型形态学改变。DNA电泳显示明显的DNA片段化。细胞周期分析显示凋亡细胞数（Sub-G1期比例）增加，S期阻滞明显。应用KSC后，Fas抗原和caspase-3 mRNA表达明显增加，Bcl-2抗原表达明显下降。KSC作用后K562/ADM细胞P53蛋白表达无明显变化。结论：KSC通过Fas-caspase-3途径诱导K562/ADM细胞凋亡。
AIM: To observe the apoptosis of K562/ADM cells induced by kappa-selenocarrageenan(KCS) and to explore its possible molecular mechanism. METHODS: MTT assay, Wright-Giemsa staining, DNA agarose gel electrophoresis and cell-cycle analysis were used for examining apoptosis in K562/ADM cells. Expression of Fas, Bcl-2 and P53 proteins was measured with Flow cytometr(FCM). RP-PCR assay was employed to detect the expression of caspase-3 mRNA. RESULTS: KCS inhibited proliferation of K562/ADM cells. Morphological typical changes of apoptosis were observed through light microscopy. DNA electrophoresis showed evident DNA fragmentation. Cell-cycle analysis indicated increased apoptotic cell population (Sub-G_1 proportion) as well as apparent S phase arrest. The expression of Fas antigens and caspase-3 mRNA significantly increased, and that of Bcl-2 antigens decreased sharply after application of KSC. There was no distinct change of the expression of P53 protein in K562/ADM cells treated with KSC. CONCLUSION: KSC induces apoptosis of K562/ADM cells via Fas-caspase-3 pathway.