SEQUENCES OF THE ESCHERICHIA-COLI UVRB GENE AND PROTEIN
SEQUENCES OF THE ESCHERICHIA-COLI UVRB GENE AND PROTEIN
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DOI：
10.1093/nar/14.6.2637
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发表时间：
1986-03-25
影响因子：
14.9
通讯作者：
SANCAR, A
中科院分区：
文献类型：
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作者：
ARIKAN, E;KULKARNI, MS;SANCAR, A
UvrB 蛋白是 E 的三个亚基之一。大肠杆菌ABC 核酸外切酶。我们已经报道了另外两个亚基 UvrA 和 UvrC 蛋白的序列。本文介绍了 UvrB 蛋白的序列。蛋白质序列由uvrB基因的DNA序列确定，并通过对UvrB蛋白质的NH2末端进行测序并分析其整体氨基酸组成来确认。 uvrB 的编码区是 2019 个碱基对，指定了由 672 个氨基酸组成的蛋白质，M 为 76,118。 UvrB 蛋白的序列与 UvrC 蛋白的序列以及 UvrA 蛋白的 ATP 结合位点显示出中等水平的同源性。在 UvrB 蛋白纯化过程中，会大量产生蛋白水解产物 UvrB*。我们发现 UvrB* 是由于从 UvrB 蛋白的 COOH 末端去除约 40 个氨基酸而产生的。 uvrB 基因具有复杂的调控特征。在51侧，编码区前面有3个启动子、一个DnaA盒和一个SOS盒。在该基因的 3' 侧，后面是 REP（重复外源回文）序列，该序列通过未知机制参与基因调控。
The UvrB protein is one of the three subunits of theE. coliABC exci-nuclease. We have reported the sequences of the other two subunits, the UvrA and UvrC proteins. In this paper the sequence of the UvrB protein is presented. The protein sequence was determined from the DNA sequence of the uvrB gene and was confirmed by sequencing the NH2-terminus of the UvrB protein and analyzing its overall amino acid composition. The coding region of uvrB is 2019 basepairs, specifying a protein of 672 amino acids and M of 76,118. The sequence of the UvrB protein shows a moderate level of homology to that of the UvrC protein and to the ATP binding site of the UvrA protein. During purification of UvrB protein a proteolytic product, UvrB*, is produced in high quantities. We find that UvrB*results from removal of about 40 amino acids from the COOH-terminus of the UvrB protein. The uvrB gene has complex regulatory features. On the 51 side, the coding region is preceded by 3 promoters, a DnaA box and an SOS box. On the 3' side the gene is followed by an REP (Repetitive Extragenic Palindrome) sequence which has been implicated in gene regulation by an unknown mechanism.