Development of a noninvasive method for detecting and monitoring the time course of Helicobacter pylori infection.
Development of a noninvasive method for detecting and monitoring the time course of Helicobacter pylori infection.
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开发一种非侵入性方法来检测和监测幽门螺杆菌感染的时间过程。
DOI：
10.1128/iai.72.9.5358-5364.2004
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发表时间：
2004
影响因子：
3.1
通讯作者：
ColemanJr,WilliamG
中科院分区：
文献类型：
--
作者：
Nyan,DougbehC;Welch,AnthonyR;Dubois,Andre;ColemanJr,WilliamG
实验性接种小鼠后幽门螺杆菌感染状态目前需要安乐死。本研究的目的是发展一种方法来跟踪H。幽门螺杆菌感染的实验动物。26只C57 BL/6雌性小鼠接种H. pyloriSydney菌株1和16只小鼠进行假接种。在大约1年的时间内，用anH对小鼠进行重复测试。从小鼠粪粒中提取的DNA的基于幽门特异性引物的PCR分析。分别在6个月和10个月处死小鼠，通过组织学、培养和PCR检测胃黏膜的感染情况。在尸检前6个月和10个月，通过PCR方法检测接种幽门螺杆菌的小鼠。分别在6个月和10个月时检测的13只小鼠中的9只（69%）和13只小鼠中的10只（77%）为阳性。所有假接种小鼠均为阴性。这两个PCR结果表明特异性为100%，灵敏度范围在69%和77%之间。相比之下，如果每天测试一次小鼠组，持续4天，则灵敏度和特异性上升至90%和100%。77%~ 85%的实验小鼠也呈H阳性。幽门螺杆菌培养组织病理学显示轻度至重度胃炎。这些发现表明，H.活小鼠中的幽门螺杆菌感染可以随时间反复评估。这种方法可以在不进行尸检的情况下确定感染的时间进程以及药物和/或疫苗的有效性。
Helicobacter pyloriinfection status following experimental inoculation of mice presently requires euthanasia. The purpose of this study was to develop a method for following the time course ofH. pyloriinfection in live experimental animals. Twenty-six C57BL/6,Helicobacter-free female mice were inoculated withH. pyloriSydney strain 1, and 16 mice were sham inoculated. The mice were repeatedly tested during a period of about 1 year with anH. pylorispecies-specific primer-based PCR analysis of DNA extracted from fecal pellets of mice. The mice were euthanized at 6 months (n= 15) and 10 months (n= 15) to determine their infection status by histology, culture, and PCR of gastric specimens.H. pylori-inoculated mice were tested via the PCR method at 6 and 10 months prior to necropsy. Nine of 13 (69%) and 10 of 13 (77%) mice tested at 6 and 10 months, respectively, were positive. All sham-inoculated mice were negative. These two PCR results suggested a specificity of 100% with a sensitivity range between 69 and 77%. In contrast, sensitivity and specificity rose to 90 and 100% if groups of mice were tested once daily for 4 days. Seventy-seven to 85% of the experimental mice were also positive forH. pyloriby culture. The histopathology demonstrated mild to severe gastritis. These findings demonstrate that the persistence or transience ofH. pyloriinfection in live mice can be repeatedly evaluated over time. This method could allow the determination of the time course of infection and the efficacy of medications and/or vaccine without necropsy.