Bombykol receptors in the silkworm moth and the fruit fly
Bombykol receptors in the silkworm moth and the fruit fly
复制标题
DOI：
10.1073/pnas.1003881107
复制
发表时间：
2010-05-18
影响因子：
11.1
通讯作者：
Leal, Walter S.
中科院分区：
文献类型：
--
作者：
Syed, Zainulabeuddin;Kopp, Artyom;Leal, Walter S.
雄蛾具有气味感受器，对性信息素的感受具有高度的敏感性和选择性。我们偶然发现，果蝇（Drosophila melanogaster）的内源性OR对蚕蛾（bombykol）的性信息素高度敏感。有趣的是，果蝇的探测器比蚕蛾的受体更敏感，尽管它的生态意义尚不清楚。通过在“空神经元”系统中的表达，我们将果蝇对家蚕酚敏感的OR鉴定为DmelOR 7a（= DmOR 7a）。该受体在天然感受器（ab 4）或空神经元系统（ab 3感受器）中表达的对家蚕酚的反应谱是不可区分的。野生型和转基因果蝇都以高灵敏度、剂量依赖性方式和快速信号终止作出反应。与此相反，相同的空神经元表达蛾bombykol受体，BmorOR 1，表现出低敏感性和缓慢的信号失活。当表达在果蝇的触角感器T1，神经元住房BmorOR 1响应的敏感性与本地触角感器在蚕蛾。通过挑战本地bombykol受体在果蝇与高剂量的另一种气味的受体响应最高的灵敏度，我们证明，缓慢的信号终止诱导过量的刺激。与锥形感器中的空神经元系统相反，感器的结构、生物化学和/或生物物理特征使得果蝇的T1类神经元系统成为蛾受体的更好替代物。
Male moths are endowed with odorant receptors (ORs) to detect species-specific sex pheromones with remarkable sensitivity and selectivity. We serendipitously discovered that an endogenous OR in the fruit fly, Drosophila melanogaster, is highly sensitive to the sex pheromone of the silkworm moth, bombykol. Intriguingly, the fruit fly detectors are more sensitive than the receptors of the silkworm moth, although its ecological significance is unknown. By expression in the "empty neuron" system, we identified the fruit fly bombykol-sensitive OR as DmelOR7a (= DmOR7a). The profiles of this receptor in response to bombykol in the native sensilla (ab4) or expressed in the empty neuron system (ab3 sensilla) are indistinguishable. Both WT and transgenic flies responded with high sensitivity, in a dose-dependent manner, and with rapid signal termination. In contrast, the same empty neuron expressing the moth bombykol receptor, BmorOR1, demonstrated low sensitivity and slow signal inactivation. When expressed in the trichoid sensilla T1 of the fruit fly, the neuron housing BmorOR1 responded with sensitivity comparable to that of the native trichoid sensilla in the silkworm moth. By challenging the native bombykol receptor in the fruit fly with high doses of another odorant to which the receptor responds with the highest sensitivity, we demonstrate that slow signal termination is induced by overdose of a stimulus. As opposed to the empty neuron system in the basiconic sensilla, the structural, biochemical, and/or biophysical features of the sensilla make the T1 trichoid system of the fly a better surrogate for the moth receptor.