TRYPANOSOMA-CRUZI INFECTION IN MICE ENHANCES THE MEMBRANE EXPRESSION OF LOW-AFFINITY FC-RECEPTORS FOR IGG AND THE RELEASE OF THEIR SOLUBLE FORMS
TRYPANOSOMA-CRUZI INFECTION IN MICE ENHANCES THE MEMBRANE EXPRESSION OF LOW-AFFINITY FC-RECEPTORS FOR IGG AND THE RELEASE OF THEIR SOLUBLE FORMS
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DOI：
10.1111/j.1365-3024.1993.tb00642.x
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发表时间：
1993-09-01
影响因子：
2.2
通讯作者：
CARLIER, Y
中科院分区：
文献类型：
--
作者：
JORGE, TA;ELBOUHDIDI, A;CARLIER, Y
应用2-4G2单抗，用流式细胞仪检测了克氏锥虫感染小鼠的FcGammaRII/III(+)细胞数和低亲和力Fc受体(FcGammaRII/III)的膜表达。分别于感染后第10、20、30、40天采集小鼠脾、肠系膜淋巴结和腹膜细胞。研究了感染小鼠体内血清中可溶性FcGammaRII/III的水平及其体外释放情况。还记录了寄生虫血症和IgG1、IgG2a和IgG2b弓形虫特异性抗体滴度。脾和肠系膜淋巴结FcGammaR(+)细胞数和细胞膜FcGammaR表达均增加，腹膜FcGammaR(+)细胞数无明显变化。脾的改变发生在感染寄生虫的早期和晚期，即检测到克氏毛滴虫特异性抗体之前和之后(第10天至40天，P.I.)。在肠系膜淋巴结中，只有在早期急性感染时才观察到这种变化，当时还没有检测到显著水平的抗体(第10天和第20天P.I.)。从第20~40d，血清、脾和淋巴结细胞培养上清液中均检测到较高水平的可溶性FcGammaR。这些结果表明，在小鼠感染急性期，在抗体应答上升之前和之后，克氏毛滴虫感染上调了FcGammaRII/III的表达和释放。
The membrane expression of low-affinity Fc receptors for IgG (FcgammaRII/III) on cells and the number of FcgammaRII/III(+) cells were studied by flow cytometry, using the 2-4G2 MoAb, in mice infected by Trypanosoma cruzi. Cells from spleen, mesenteric lymph nodes and peritoneum were collected on days 10, 20, 30 and 40 post infection (p.i.). The in vivo serum level of soluble FcgammaRII/III, as well as its in vitro release by cells from infected mice were studied. Parasitaemia and IgG1, IgG2a andIgG2b T. cruzi-specific antibody titres were also recorded. Both the expression of FcgammaR on cell membrane and the absolute number of FcgammaR(+) cells increased in spleen and in mesenteric lymph nodes, but not in peritoneum. The modifications in spleen occurred in the early and late parasitaemic phase of infection, i.e., before and after detection of T. cruzi-specific antibodies (from day 10 to 40 p.i.). In mesenteric lymph nodes, the variations were observed only in the early acute infection, when antibodies were not yet detectable at significant levels (on days 10 and 20 p.i.). Higher levels of soluble FcgammaR were detected in sera and in culture supernatants of spleen and lymph node cells from day 20 to 40 p.i. These results show that T. cruzi infection in mice upregulates the expression and the release of FcgammaRII/III, in the acu te phase of infection, before as well as after the rise of antibody response.