Purification and characterization of ATBP, a novel protein that binds to A/T stretches in three segments of the Sarcophaga lectin gene.
Purification and characterization of ATBP, a novel protein that binds to A/T stretches in three segments of the Sarcophaga lectin gene.
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ATBP 的纯化和表征，ATBP 是一种与 Sarcophaga 凝集素基因的三个片段中的 A/T 片段结合的新型蛋白质。
DOI：
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发表时间：
1994
期刊：
影响因子：
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通讯作者：
Shunji Natori
中科院分区：
文献类型：
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作者：
Mayumi Matsui;Ayako Kobayashi;T. Kubo;Shunji Natori
从NIH-Sape-4细胞的核提取物中纯化了一种与肉蝇属凝集素基因结合的DNA结合蛋白ATBP（A/T-延伸结合蛋白）。在变性条件下测定的ATBP的分子量为53 kDa，但其天然分子量估计凝胶过滤色谱法为430 kDa，这表明它是一个八聚体的53 kDa亚基。该蛋白质与来自Sarcophaga凝集素基因的至少三个DNA片段结合，其中两个在5 '上游区域，另一个在含有内含子的区域。这些片段非常富含AT并且镶嵌有A或T残基的延伸（A/T延伸）。ATBP对poly[d（A-T）]具有亲和性。这些结果表明，ATBP结合到该基因的三个DNA片段中的A/T延伸。此外，较长的DNA被发现更有效地结合，这表明一个八聚体的53 kDa亚基的DNA片段具有多个结合位点，并需要一个相对较长的DNA序列的结合。
A DNA-binding protein for the Sarcophaga lectin gene, ATBP (A/T-stretches-binding protein) was purified to homogeneity from the nuclear extract of NIH-Sape-4 cells. The molecular mass of ATBP determined under denaturing conditions was 53 kDa, but its native molecular mass estimated by gel-filtration chromatography was 430 kDa, suggesting that it is an octamer of the 53-kDa subunit. This protein bound to at least three DNA fragments from the Sarcophaga lectin gene, two of them are in the 5'-upstream region and the other is in a region containing an intron. These fragments are very AT rich and inlaid with stretches of A or T residues (A/T stretches). ATBP was found to have affinity for poly[d(A-T)]. These results suggest that ATBP binds to A/T stretches in the three DNA fragments from this gene. Furthermore, longer DNA was found to be bound more effectively, suggesting that an octamer of the 53-kDa subunit has multiple binding sites for the DNA fragments and requires a relatively long DNA sequence for binding.
影响因子：
56.9
作者：
BODE, J;KOHWI, Y;KOHWISHIGEMATSU, T
通讯作者：
KOHWISHIGEMATSU, T