MONOREACTIVE HIGH-AFFINITY AND POLYREACTIVE LOW AFFINITY RHEUMATOID FACTORS ARE PRODUCED BY CD5+ B-CELLS FROM PATIENTS WITH RHEUMATOID-ARTHRITIS
MONOREACTIVE HIGH-AFFINITY AND POLYREACTIVE LOW AFFINITY RHEUMATOID FACTORS ARE PRODUCED BY CD5+ B-CELLS FROM PATIENTS WITH RHEUMATOID-ARTHRITIS
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DOI：
10.1084/jem.168.6.1979
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发表时间：
1988-12-01
影响因子：
15.3
通讯作者：
NOTKINS, AL
中科院分区：
文献类型：
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作者：
BURASTERO, SE;CASALI, P;NOTKINS, AL
在类风湿性关节炎（RA）患者中，循环中的CD 5 + B淋巴细胞（而非CD 5- B淋巴细胞）数量和大小增加，以活化状态存在，自发增殖，并分泌与IgG的Fc片段结合的IG。通过构建CD 5 + B淋巴细胞连续分泌单克隆抗体的细胞系，测定了这些抗体的性质和解离常数（Kd）。产生了两种具有离散反应性的类风湿因子（RF）。第一种类型是多反应性的，并且以相对低的亲和力（Kd，10-5 mol/L）结合IgG的Fc片段。这些抗体与健康受试者的CD 5 + B细胞产生的抗体相似。第二种类型的RF是单反应性的，并且以更高的亲和力（Kd，10-7 mol/L）结合IgG的Fc片段。这些后一种自身抗体由RA患者的CD 5 + B细胞产生，而不是由健康受试者产生。需要长期的纵向研究来确定这两种类型的RF在RA发病机制中的作用。
In patients with rheumatoid arthritis (RA), circulating CD5+ B lymphocytes, but not CD5- B lymphocytes, are increased in number and size, exist in an activated state, spontaneously proliferate, and secrete Ig that binds to the Fc fragment of IgG. By constructing continuous mAb-secreting cell lines from CD5+ B lymphocytes, the properties and dissociation constants (Kd) of these antibodies were determined. Two types of rheumatoid factors (RFs) with discrete reactivities were produced. The first type is polyreactive and binds with relatively low affinity (Kd, 10-5 mol/liter) to the Fc fragment of IgG. These antibodies are similar to those produced by CD5+ B cells from healthy subjects. The second type of RF is monoreactive and binds with higher affinity (Kd, 10-7 mol/liter) to the Fc fragment of IgG. These latter autoantibodies are produced by CD5+ B cells of RA patients, but not healthy subjects. Long-term longitudinal studies are needed to determine the role of these two types of RFs in the pathogenesis of RA.