Dark septate endophytic pleosporalean genera from semiarid areas.
Dark septate endophytic pleosporalean genera from semiarid areas.
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DOI：
10.3767/003158515x687669
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发表时间：
2015-12
期刊：
影响因子：
9.1
通讯作者：
Crous PW
中科院分区：
文献类型：
--
作者：
Knapp DG;Kovács GM;Zajta E;Groenewald JZ;Crous PW
暗隔膜内生菌(DSE)作为根部定植真菌广泛分布于世界各地，常见于强非生物胁迫环境中。DSE不是一个分类单元，而是由多个子囊菌目的真菌组成。在这项研究中，我们调查了三个以前在半干旱沙质草原上发现的属于毛孢子目的未确定的DSE谱系。在分子系统发育研究中，共扩增并测序了7个基因座(ITS、部分18SRRNA、28SRRNA、肌动蛋白、钙调素、转录延伸因子1-α和B-微管蛋白基因)。根据形态和分子系统发育分析，这些菌株代表了毛孢子目中的3个新属，即水霉属、黄霉属和暗色霉属，其中有8个新种。分子数据显示，单型水生真菌属于Morosphaeriaceae，单型黄霉代表与马尾草科有关的一个确定的莎草属谱系，Darkside a有6个新种，与Lentitheciaceae有亲缘关系。在这项研究中，我们测试了许多诱导孢子形成的条件，并首次成功地诱导了几个DSE形成它们的有性形态。
Dark septate endophytes (DSE) are distributed worldwide as root-colonising fungi, and frequent in environments with strong abiotic stress. DSE is not a taxon, but constitutes numerous fungal taxa belonging to several orders of Ascomycota. In this study we investigate three unidentified DSE lineages belonging to Pleosporales that were found previously in semiarid sandy grasslands. For molecular phylogenetic studies seven loci (ITS, partial 18S nrRNA, 28S nrRNA, actin, calmodulin, transcription-elongation factor 1- α and ß -tubulin genes) were amplified and sequenced. Based on morphology and the resulting molecular phylogeny these isolates were found to represent three novel genera within the Pleosporales, namely Aquilomyces, Flavomyces and Darksidea, with eight novel species. Molecular data revealed that monotypic Aquilomyces belongs to Morosphaeriaceae, monotypic Flavomyces represents an incertae sedis lineage related to Massarinaceae, and Darksidea, with six new species, is allied to the Lentitheciaceae. During this study we tested numerous conditions to induce sporulation, and managed for the first time to induce several DSE to form their sexual morphs.