Contrasting effects of HC-Pro and 2b viral suppressors from Sugarcane mosaic virus and Tomato aspermy cucumovirus on the accumulation of siRNAs.
Contrasting effects of HC-Pro and 2b viral suppressors from Sugarcane mosaic virus and Tomato aspermy cucumovirus on the accumulation of siRNAs.
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DOI：
10.1016/j.virol.2007.12.045
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发表时间：
2008-05
期刊：
影响因子：
3.7
通讯作者：
Xiaoming Zhang;P. Du;Lu Lu-Lu;Q. Xiao;Wenjuan Wang;Xuesong Cao;B. Ren;Chunhong Wei;Yi Li
中科院分区：
文献类型：
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作者：
Xiaoming Zhang;P. Du;Lu Lu-Lu;Q. Xiao;Wenjuan Wang;Xuesong Cao;B. Ren;Chunhong Wei;Yi Li
RNA沉默和沉默的抑制是宿主和病毒相互作用的防御和反防御。本文探讨了甘蔗花叶病毒（SCMV）编码的HC-Pro对抑制RNA沉默的功能影响。siRNA北方印迹分析表明，SCMV的复制受宿主RNA沉默机制的调控。共表达实验表明，SCMV编码的HC-Pro抑制正义RNA和dsRNA诱导的RNA沉默。瞬时RNA沉默实验表明，HC-Pro下调3′端二级siRNA的积累，但不影响5′端二级和初级siRNA的积累。同时，番茄无芒黄瓜病毒（Tomato aspermy cucumovirus，Tav）2b基因显著下调了5′端次级siRNA的积累。重要的是，我们发现HC-Pro和Tav 2b下调RDR 6 mRNA的积累。因此，HC-Pro，一种由SCMV编码的RNA沉默抑制子，调节不同siRNA的积累，并且在RNA沉默途径中具有多于一个靶标。
RNA silencing and suppression of silencing are host and virus interactions for defense and counter-defense. Here, we explored the function effect of HC-Pro encoded by Sugarcane mosaic virus (SCMV) on the suppression of RNA silencing. siRNA northern blotting assay indicated that the replication of SCMV was regulated by host RNA silencing machinery. Co-expression assay demonstrated that the HC-Pro encoded by SCMV suppressed the RNA silencing induced by sense RNA and dsRNA. Transitive RNA silencing assay showed that HC-Pro down-regulated the accumulation of 3′ secondary siRNA, but not 5′ secondary and primary siRNA. Meanwhile, the 2b gene of Tomato aspermy cucumovirus (Tav) evidently down-regulated the accumulation of 5′ secondary siRNA. Importantly, we found that HC-Pro and Tav2b down-regulated the accumulation of RDR6 mRNA. Thus, HC-Pro, an RNA silencing suppressor encoded by SCMV, regulates the accumulation of different siRNAs and has more than one target in the RNA silencing pathway.