Slit is the midline repellent for the robe receptor in Drosophila
Slit is the midline repellent for the robe receptor in Drosophila
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DOI：
10.1016/s0092-8674(00)80589-9
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发表时间：
1999-03-19
期刊：
影响因子：
64.5
通讯作者：
Goodman, CS
中科院分区：
文献类型：
--
作者：
Kidd, T;Bland, KS;Goodman, CS
以前的研究表明，Roundabout（Robo）是生长锥上的排斥性导向受体，与未知的中线配体结合。在这里，我们提出的遗传证据表明，狭缝是中线长袍配体;配套文件提出的生化证据表明，狭缝结合长袍。Slit是由中线胶质细胞表达的大的细胞外基质蛋白。在狭缝突变体中，生长锥进入中线但从未离开它;它们在中线处异常地继续表达高水平的长袍。狭缝和长袍显示剂量敏感的遗传相互作用，表明它们在相同的途径中发挥作用。肌前体远离中线的迁移也需要狭缝。Slit似乎起着控制中线轴突交叉的短程排斥剂和控制中胚层远离中线迁移的长程化学排斥剂的作用。
Previous studies suggested that Roundabout (Robo) is a repulsive guidance receptor on growth cones that binds to an unknown midline ligand. Here we present genetic evidence that Slit is the midline Robe ligand; a companion paper presents biochemical evidence that Slit binds Robe. Slit is a large extracellular matrix protein expressed by midline glia. In slit mutants, growth cones enter the midline but never leave it; they abnormally continue to express high levels of Robe while at the midline. slit and robe display dosage-sensitive genetic interactions, indicating that they function in the same pathway. slit is also required for migration of muscle precursors away from the midline. Slit appears to function as a short-range repellent controlling axon crossing of the midline and as a long-range chemorepellent controlling mesoderm migration away from the midline.