Characterization and expression pattern of the trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase gene families in Populus
Characterization and expression pattern of the trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase gene families in Populus
复制标题
杨属海藻糖-6-磷酸合酶和海藻糖-6-磷酸磷酸酶基因家族的特征和表达模式。
DOI：
10.1016/j.ijbiomac.2021.07.096
复制
发表时间：
2021-07-22
影响因子：
8.2
通讯作者：
Chen, Zhong
中科院分区：
文献类型：
--
作者：
Gao, Yuhan;Yang, Xiaoyu;Chen, Zhong
海藻糖在植物代谢、生长发育和抗逆性中起着重要作用。海藻糖-6-磷酸合酶基因（TPS）和海藻糖-6-磷酸磷酸酶基因（TPP）是海藻糖合成的关键基因。杨树是一种重要的多年生木本植物，其TPS和TPP家族的系统全基因组分析是有限的。在本研究中，13 PtTPS和10 PtTPP基因在杨树基因组中被确定。系统进化分析表明，PtTPS和PtTPP基因均分为两个亚家族，每个亚家族的基因成员具有高度保守的内含子结构。顺式作用元件分析表明，PtTPS和PtTPP基因参与植物激素和环境胁迫反应。表达谱还发现PtTPSs和PtTPPs在盐胁迫、寒冷、机械损伤、水杨酸和茉莉酸甲酯处理中表达不同。RT-PCR结果表明，PtTPSs和PtTPPs在杨树雌雄花芽发育的7个阶段都有特异性表达。本研究不仅为揭示PtTPS和PtTPP基因家族在白杨海藻糖调控中的功能奠定了基础，也为其它多年生植物海藻糖相关研究提供了参考。
Trehalose plays an important role in plant metabolism, growth development, and stress tolerance. Trehalose-6phosphate synthase gene (TPS) and trehalose-6-phosphate phosphatase gene (TPP) are vital for the synthesis of trehalose. Populus is a prominent perennial woody plant, in which systematic genome-wide analysis of the TPS and TPP family is limited. In this study, 13 PtTPS and 10 PtTPP genes were identified in the Populus genome. Phylogenetic analysis indicated PtTPS and PtTPP genes were both divided into two subfamilies, and gene members of each subfamily have highly conserved intron structures. Analysis of cis-acting elements showed that PtTPS and PtTPP genes were involved in plant hormones and environmental stress responses. Expression profiles also found PtTPSs and PtTPPs expressed differently in response to salt stress, cold, mechanical damage, salicylic acid, and methyl jasmonate treatment. Furthermore, reverse transcription quantitative real-time PCR results found PtTPSs and PtTPPs displayed a specific expression pattern in the seven developmental stages of Populus male and female floral buds. This work will not only lead a foundation on reveal the functions of PtTPS and PtTPP gene families in trehalose regulation of poplar but also provide references to related trehalose research in other perennial plants.