1.Pull-down method to access the cell surface receptor for Toxoplasma gondii.
1.Pull-down method to access the cell surface receptor for Toxoplasma gondii.
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1.Pull-down法接触弓形虫细胞表面受体。
DOI：
10.1016/j.parint.2016.08.013
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Kato K
中科院分区：
文献类型：
--
作者：
Gong H;Kobayashi K;Sugi T;Takemae H;Horimoto T;Xuan X;Akashi H;Kato K
蛋白X在弓形虫表面表达，被认为是与宿主分子相互作用的蛋白，以GST重组蛋白的形式表达，连接到Sepharose4B上，用于拖拉生物素标记的293T细胞。用2D-PAGE和Western blotting对产物进行分析。质谱仪显示，凝胶中的反应斑点是热休克蛋白。
Protein X, which is expressed on the surface ofToxoplasmaand is thought to interact with host molecules, was expressed as a GST recombinant protein, conjugated to sepharose 4B, and used to pull down biotin-labeled 293T cells. The product was analysis by 2D-PAGE and Western blotting. Mass spectrometry revealed the reacted spots from the gel to be heat shock proteins.