Flexible needle-type Microbiosensor for real-time monitoring traditional acupuncture-mediated adenosine release In vivo
Flexible needle-type Microbiosensor for real-time monitoring traditional acupuncture-mediated adenosine release In vivo
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DOI：
10.1016/j.bios.2023.115383
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发表时间：
2023-05-17
影响因子：
12.6
通讯作者：
Fang，Yuxin
中科院分区：
文献类型：
--
作者：
Chang，Hongen;Huo，Mingzhu;Fang，Yuxin
腺苷（ADO）快速信号传导在数秒的时间范围内调节生理和病理过程，包括针灸的疗效。然而，标准的监测策略受到时间分辨率差的限制。在此，已经开发了一种能够监测响应于针灸的体内真实的释放ADO的可植入针型微传感器。电催化普鲁士蓝纳米粒子，固定化的多酶系统，和选择性渗透聚邻苯二胺为基础的膜被用于顺序修饰的电极的传感区域。所得到的传感器可以响应于极低水平的施加电位（−0.05 VvsAg/AgCl）来执行ADO水平的安培测量。该微传感器在0-50 μM的线性范围内工作，灵敏度为1.1 nA/μM，响应时间小于5 s。重要的是，该传感器还表现出良好的重现性和高选择性。对于在体动物研究，微传感器用于连续评估ST 36（足三里）穴位时，该穴位受到捻转-旋转针刺手法的瞬时ADO释放。受益于优越的上级传感器体内性能和稳定性，可以首次证明穿刺诱导的ADO释放的可变性与影响临床益处的刺激强度水平之间的正相关性。总之，这些结果突出了一个强大的方法来分析在体内的生理效应的针灸，扩大应用领域的微纳米传感器技术在一个快速的时间尺度。
Rapid adenosine (ADO) signaling, on the time frame of seconds, regulates physiological and pathological processes, including the therapeutic efficacy of acupuncture. Nevertheless, standard monitoring strategies are limited by poor temporal resolution. Herein, an implantable needle-type microsensor capable of monitoring ADO release in vivo in response to acupuncture in real time has been developed. Electrocatalytic Prussian Blue nanoparticles, an immobilized multienzyme system, and a permselective poly-o-phenylenediamine-based membrane were used for the sequential modification of the sensing region of the electrode. The resultant sensor can perform amperometric measurements of ADO levels in response to a very low level of applied potential (−0.05 VvsAg/AgCl). This microsensor also functioned across a broad linear range (0–50 μM) and exhibited good sensitivity (1.1 nA/μM) with a rapid response time of under 5 s. Importantly, the sensor also exhibited good reproducibility and high selectivity. For in vivo animal studies, the microsensor was employed for the continuous assessment of instantaneous ADO release at the ST36 (Zusanli) acupoint when this acupoint was subjected to twirling-rotating acupuncture manipulation. Benefiting from superior sensor in vivo performance and stability, the positive correlation between the variability in acupuncture-induced ADO release and the stimulus intensity levels that affect the clinical benefit can be demonstrated for the first time. Overall, these results highlight a powerful approach to analyzing the in vivo physiological effects of acupuncture, expanding application realm of micro-nano sensor technology on a fast time scale.