Monitor human cytomegalovirus active infection by two quantitativly detect methods
Monitor human cytomegalovirus active infection by two quantitativly detect methods
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发表时间：
2002
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通讯作者：
Zhao Zhe
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作者：
Zhao Zhe
目的应用两种定量方法检测并比较不同人群人巨细胞病毒活动性感染。方法对106例204例不同人群的血液透析患者进行两种方法的检测。结果血浆中HCMVpp 65抗原血症与定量PCR检测的符合率为84.8%，相关系数为t =0.861，PNML中HCMVpp 65抗原血症与定量PCR检测的符合率为79.8%。6例骨髓移植受者中，5例（81.7%）在移植后30~50天PMNLs和血浆中HCMV DNA拷贝数均升高。PMNLs中HCMV DNA拷贝数高于血浆中HCMV DNA拷贝数。无症状组HCMV DNA拷贝数（7.71× 103/ml）和HCMV pp 65阳性细胞数（104/2× 105）均高于无症状组（1.53× 102/ml和27/2× 105）。结论定量PCR在HCMV病毒载量较低时敏感性较高，而HCMV pp 65抗原血症检测对HCMV疾病的预测价值更高，对抗病毒治疗的敏感性更高。两种方法同时检测HCMV活动性感染是有效的。
Objective Detect and compare human cytomegalovirus active infection in different people by two quantitative methods. Methods 204 blood speciments from different groups (106 cases)were tested with two methods. Results The concordance of HCMV pp65 antigenemia and quantitative PCR assay in plasma is 84.8%, correlation coefficien t =0.861; the concordance of HCMV pp65 antigenemia and quantitative PCR assay in PNMLs is 79.8%. In 6 bone marrow transplant recipients, 5 patients′ HCMV DNA copy number, both in the PMNLs and plasma (81.7%), were in increased 30～50 days after transplantation. HCMV DNAcopies in PMNLs was higher than that in plasma. Either immunocompromised or immunocompetent group, the mean of HCMV DNA copy number (7.71×10 3/ml) and HCMV pp65 positive cells(104/2×10 5) in asymptomatic patients are higher than in asymptomatic patients(1.53×10 2/ml, 27/2×10 5). Conclusion The sensitivity of quantitative PCR is higher when HCMV viral load is low, but HCMV pp65 antigenemia assay has higher predictive value of HCMV disease and more sensitive to antiviral treatment than HCMV DNA detection. Detecting HCMV active infection by the two methods at the same time is effecitively.