MicroRNA profiling of antler stem cells in potentiated and dormant states and their potential roles in antler regeneration
MicroRNA profiling of antler stem cells in potentiated and dormant states and their potential roles in antler regeneration
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鹿角干细胞在增强和休眠状态下的 MicroRNA 分析及其在鹿角再生中的潜在作用
DOI：
10.1007/s00438-015-1158-8
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发表时间：
2016-04-01
影响因子：
3.1
通讯作者：
Li, Chunyi
中科院分区：
文献类型：
--
作者：
Ba, Hengxing;Wang, Datao;Li, Chunyi
microRNAs（miRNAs）能够在转录后水平有效调控基因表达，在组织的生长、发育和再生中发挥重要作用。我们以前的研究表明，鹿茸再生是一个基于干细胞的过程，鹿茸干细胞驻留在鹿茸蒂的骨膜，永久性的骨突起，鹿茸再生发生。鹿角是唯一可以完全再生的哺乳动物器官，因此为鉴定参与器官再生的miRNA提供了独特的机会。在本研究中，我们使用新一代测序技术对来自增强或休眠的椎弓根骨膜的干细胞的miRNA进行测序。鉴定了保守的和20种鹿特异性miRNA的群体。这些保守的miRNA来自88种动物的453个同源发夹前体，并进一步分为167个miRNA家族。其中，miR-296是胚胎干细胞特异性的。增强过程导致保守miRNA的显著调节（>±2倍，q值<0.05）; 8个miRNA转录物下调，6个上调。发现几个GO生物学过程和Wnt、MAPK和TGF-β信号通路作为生茸干细胞增强过程的一部分被上调。这项研究已经确定了与休眠或增强的鹿茸干细胞相关的miRNAs，并确定了一些靶miRNAs，以进一步研究它们在哺乳动物器官再生中的作用。
MicroRNAs (miRNAs) can effectively regulate gene expression at the post-transcriptional level and play a critical role in tissue growth, development and regeneration. Our previous studies showed that antler regeneration is a stem cell-based process and antler stem cells reside in the periosteum of a pedicle, the permanent bony protuberance, from which antler regeneration takes place. Antlers are the only mammalian organ that can fully regenerate and hence provide a unique opportunity to identify miRNAs that are involved in organ regeneration. In the present study, we used next generation sequencing technology sequenced miRNAs of the stem cells derived from either the potentiated or the dormant pedicle periosteum. A population of both conserved and 20 deer-specific miRNAs was identified. These conserved miRNAs were derived from 453 homologous hairpin precursors across 88 animal species, and were further grouped into 167 miRNA families. Among them, the miR-296 is embryonic stem cell-specific. The potentiation process resulted in the significant regulation (>±2 Fold,qvalue <0.05) of conserved miRNAs; 8 miRNA transcripts were down- and 6 up-regulated. Several GO biology processes and the Wnt, MAPK and TGF-beta signaling pathways were found to be up-regulated as part of antlerogenic stem cell potentiation process. This research has identified miRNAs that are associated either with the dormant or the potentiated antler stem cells and identified some target miRNAs for further research into their role played in mammalian organ regeneration.