OVINE TROPHOBLAST PROTEIN-1, AN EARLY SECRETED BLASTOCYST PROTEIN, BINDS SPECIFICALLY TO UTERINE ENDOMETRIUM AND AFFECTS PROTEIN-SYNTHESIS
OVINE TROPHOBLAST PROTEIN-1, AN EARLY SECRETED BLASTOCYST PROTEIN, BINDS SPECIFICALLY TO UTERINE ENDOMETRIUM AND AFFECTS PROTEIN-SYNTHESIS
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DOI：
10.1210/endo-114-1-120
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发表时间：
1984-01-01
期刊：
影响因子：
4.8
通讯作者：
ROBERTS, RM
中科院分区：
文献类型：
--
作者：
GODKIN, JD;BAZER, FW;ROBERTS, RM
绵羊滋养层蛋白-1 (oTP-1) 是绵羊囊胚的早期分泌蛋白，在体外培养第 14-16 天受孕 24 小时后纯化。通过免疫细胞化学方法测定了怀孕第 16 天绵羊子宫中 oTP-1 的定位。该蛋白质与伸长囊胚的滋养外胚层细胞以及母体子宫的表面和上腺上皮相关。以高亲和力(Kd＝约2×10-10M)结合oTP-1的受体存在于源自第12天非妊娠和不发情母羊的子宫内膜匀浆的粗制膜制剂中。将 125I 标记的 oTP-1 输注到第 12 天未怀孕的母羊体内表明，大部分放射性保留在子宫中，只有极少量的完整蛋白质似乎进入了母体脉管系统。与黄体、卵巢或其他测试组织没有显着关联。 oTP-1 无法与绵羊 PRL [催乳素] 竞争兔乳腺受体，也无法与 hCG [人绒毛膜促性腺激素] 或牛 LH [黄体生成素] 竞争绵羊黄体细胞受体，并且 oTP-1 不会刺激来自第 12 天循环母羊的分散黄体细胞产生黄体酮。将来自第12天非怀孕母羊的子宫内膜外植体与5μg/ml oTP-1一起温育导致蛋白质释放到培养基中的速率增加。二维聚丙烯酰胺凝胶电泳显示，oTP-1 的存在选择性地刺激了 6 种多肽的合成。显然，oTP-1 作用于母体子宫内膜。 oTP-1 与子宫内膜的相互作用可能引起母体反应，有助于绵羊妊娠的维持。
Ovine trophoblastic protein-1 (oTP-1), an early secretory protein of the sheep blastocyst, was purified after culturing day 14-16 conceptuses for 24 h in vitro. The localization of oTP-1 in the pregnant day 16 sheep uterus was determined immunocytochemically. The protein was associated with trophectoderm cells of the elongated blastocyst and with the surface and upper glandular epithelium of the maternal uterus. Receptors that bound oTP-1 with high affinity (Kd = .apprx. 2 .times. 10-10 M) were present in crude membrane preparations derived from homogenates of endometria from day 12 nonpregnant and anestrous ewes. Uterine infusion of 125I-labeled oTP-1 into day 12 nonpregnant ewes showed that the majority of the radioactivity was retained in the uterus, and only very small amounts of intact protein appeared to enter the maternal vasculature. There was no significant association with the corpora lutea, ovaries or other tissues tested. oTP-1 failed to compete with ovine PRL [prolactin] for rabbit mammary gland receptors or with hCG [human chroionic gonadtorpin] or bovine LH [luteinizing hormone] for sheep luteal cell receptors, and the oTP-1 did not stimulate progesterone production by dispersed luteal cells from day 12 cycling ewes. Incubation of endometrial explants from day 12 nonpregnant ewes with 5 .mu.g/ml oTP-1 resulted in increased rates of protein release into the medium. Two-dimensional polyacrylamide gel electrophoresis revealed that the synthesis of 6 polypeptides was stimulated selectively by the presence of oTP-1. Evidently, oTP-1 acts on the maternal endometrium. The interaction of oTP-1 with uterine endometrium may elicit maternal responses which contribute to the maintenance of pregnancy in the sheep.