Gold nanoparticle synthesis in contact lenses for drug-less ocular cystinosis treatment
Gold nanoparticle synthesis in contact lenses for drug-less ocular cystinosis treatment
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DOI：
10.1016/j.ejpb.2021.05.019
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发表时间：
2021-05-27
影响因子：
4.9
通讯作者：
Chauhan, Anuj
中科院分区：
文献类型：
--
作者：
Liu, Zhen;Kompella, Uday B.;Chauhan, Anuj
目的：为了开发负载金纳米颗粒的接触透镜（“Goldin透镜”），以在金纳米颗粒（GNP）的表面上结合大量胱氨酸，用于胱氨酸病治疗，这是由于胱氨酸和金之间的反应。研究方法：Goldin透镜通过在预成型接触透镜基质内合成GNP来制造，首先用金前体装载透镜（Moist和TrueEye），然后还原（用硼氢化钠或柠檬酸三钠）成金原子，金原子在聚合物基质内成核成GNP。通过SEM、XRD、UV-Vis光谱对透镜进行表征，并通过直接测量质量来确定负载在透镜中的GNP的质量。结果：结果表明，接触透镜中的金负载量与金前体浓度和制造过程的重复次数呈线性增加。更强的还原剂硼氢化钠导致更高的金负载，由于还原剂进入透镜的更高扩散率，Moist透镜中的负载更高。然而，GNP的尺寸较小，并且在TruEye Goldin透镜中相对单分散，导致24小时内胱氨酸摄取较高，为47 μ g/透镜（相对于Moist Goldin透镜的33 μ g/透镜）。然而，这种吸收率较高的湿润GoldiLens（8.25与2.35微克/小时），最大吸收发生在一个小时（与五小时）。结论：一种方法制造GoldinLens，其中小的金纳米粒子被困在隐形眼镜，已经开发了无药胱氨酸治疗。镜片从周围环境中提取胱氨酸分子，TrueEye GoldinLens在程度上上级，而Moist GoldinLens在胱氨酸去除率上上级。本研究的GoldinLenses可用于无药物胱氨酸清除胱氨酸病治疗，每次佩戴1小时或5小时。
Purpose: To develop gold nanoparticles-loaded contact lens ("GoldinLens") to bind a significant mass of cystine on the surface of the gold nanoparticles (GNPs) for cystinosis treatment due to the reaction between cystine and gold. Methods: The GoldinLens was manufactured by synthesizing GNPs inside the preformed contact lens matrix by first loading the lenses (Moist and TrueEye) with gold precursor followed by reduction (with sodium borohydride or trisodium citrate) to gold atoms, which nucleated to GNPs inside the polymeric matrix. The lenses were characterized by SEM, XRD, UV-Vis spectroscopy and mass of GNPs loaded in the lens was determined by direct measurement of mass. Manufactured lenses were soaked in cystine solution for cystine uptake in vitro.Results: Results show that gold loading in the contact lens increases linearly with gold precursor concentration and number of repetitions of the manufacturing process. The stronger reducing agent sodium borohydride resulted in higher gold loading, with the loading being higher in the Moist lenses due to higher diffusivity of the reducing agent into the lens. However, GNPs were smaller in size and relatively monodispersed in TruEye GoldinLens, resulting in higher cystine uptake of 47 mu g/lens over 24 h (vs. 33 mu g/lens for Moist GoldinLens). However, the rate of this uptake was higher for Moist GoldiLens (8.25 vs. 2.35 mu g/h), with the maximum uptake occurring in one hour (vs. five hours).Conclusion: A method for manufacturing GoldinLens, wherein small gold nanoparticles are trapped in contact lenses, has been developed for drugless cystinosis treatment. The lenses withdraw cystine molecules from the surrounding milieu, with the TrueEye GoldinLens being superior for the extent of, while Moist GoldinLens is superior for rate of cystine removal. GoldinLenses of this study can be used for drugless cystine removal cystinosis treatment with one- or five-hour wear at a time.