Isolation and characterization of avian influenza viruses, including highly pathogenic H5N1, from poultry in live bird markets in Hanoi, Vietnam, in 2001
Isolation and characterization of avian influenza viruses, including highly pathogenic H5N1, from poultry in live bird markets in Hanoi, Vietnam, in 2001
复制标题
DOI：
10.1128/jvi.79.7.4201-4212.2005
复制
发表时间：
2005-04-01
影响因子：
5.4
通讯作者：
Katz, JM
中科院分区：
文献类型：
--
作者：
Nguyen, DC;Uyeki, TM;Katz, JM
自1997年以来，高致病性H5N1病毒的爆发和H9N2病毒在亚洲家禽中的传播对公众健康构成了威胁。为了更好地了解禽流感病毒 (AIV) 在亚洲向人类传播的程度，我们于 2001 年 10 月在越南河内的活禽市场 (LBM) 进行了一项横断面病毒学研究。以 10 LBM 采集了 189 只禽类样本和 18 个环境样本。从健康鸭子中分离出四种甲型流感病毒 H4N6 (n = 1)、H5N2 (n = 1) 和 H9N3 (n = 2) 亚型，分离频率超过 30%。从健康鹅身上分离出两种 H5N1 病毒。这些H5N1病毒的血凝素（HA）基因在切割位点具有多个碱性氨基酸基序，对于实验感染的鸡来说是HP，因此被定性为HP AIV。这些HA基因与这一时期在亚洲分离的其他H5NI病毒的基因具有高度的氨基酸同一性，但它们在基因上与2004年初疫情期间从越南家禽和人类分离的H5N1病毒不同。这些病毒对于实验感染的鸭子、小鼠或雪貂来说毒性并不高。这些结果表明，与在香港和中国大陆分离出的病毒具有相似特性的 HP H5NI 病毒早在 2001 年就在越南传播，表明在这些亚洲国家传播的 H5N1 病毒有一个共同来源，并为更好地了解最近在亚洲广泛出现的 HP H5NI 病毒提供了一个框架。
Since 1997, outbreaks of highly pathogenic (HP) H5N1 and circulation of H9N2 viruses among domestic poultry in Asia have posed a threat to public health. To better understand the extent of transmission of avian influenza viruses (AIV) to humans in Asia, we conducted a cross-sectional virologic study in live bird markets (LBM) in Hanoi, Vietnam, in October 2001. Specimens from 189 birds and 18 environmental samples were collected at 10 LBM. Four influenza A viruses of the H4N6 (n = 1), H5N2 (n = 1), and H9N3 (n = 2) subtypes were isolated from healthy ducks for an isolation frequency of over 30 % from this species. Two H5N1 viruses were isolated from healthy geese. The hemagglutinin (HA) genes of these H5N1 viruses possessed multiple basic amino acid motifs at the cleavage site, were HP for experimentally infected chickens, and were thus characterized as HP AIV. These HA genes shared high amino acid identities with genes of other H5NI viruses isolated in Asia during this period, but they were genetically distinct from those of H5N1 viruses isolated from poultry and humans in Vietnam during the early 2004 outbreaks. These viruses were not highly virulent for experimentally infected ducks, mice, or ferrets. These results establish that HP H5NI viruses with properties similar to viruses isolated in Hong Kong and mainland China circulated in Vietnam as early as 2001, suggest a common source for H5N1 viruses circulating in these Asian countries, and provide a framework to better understand the recent widespread emergence of HP H5NI viruses in Asia.