Establishment of group-specific PCR primers for the identification of freshwater macroinvertebrates
Establishment of group-specific PCR primers for the identification of freshwater macroinvertebrates
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DOI：
10.1007/s12686-013-9998-1
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发表时间：
2013-12-01
影响因子：
1.1
通讯作者：
Gergs, Rene
中科院分区：
文献类型：
--
作者：
Koester, Meike;Classen, Silke;Gergs, Rene
分析物种群落和食物网的多样性对于更好地了解生态系统至关重要，基于PCR的方法通常用于分类鉴定。在这里，我们描述了21组特异性引物的发展，以扩增短节的基因编码核核糖体DNA从几个淡水无脊椎动物类群作为一种潜在的方法来识别猎物的无脊椎动物捕食者，指定的130类群的淡水大型无脊椎动物。我们的引物涵盖了广泛的水生无脊椎动物类群，并可能提供一个有用的工具，用于检测混合DNA样品中的目标群体，使它们也适合于在饮食样品中的猎物物种识别。
Analysis of the diversity of the species communities and food webs is essential for a better understanding of the ecosystem and PCR based methods are often used for taxonomic identifications. Here, we describe the development of 21 group-specific primers to amplify short sections of genes encoding nuclear ribosomal DNA from several freshwater invertebrate taxa as a potential approach to identify the prey of invertebrate predators, specified on 130 taxa of freshwater macroinvertebrates. Our primers cover a broad range of aquatic invertebrate taxa and may provide a useful tool for detecting target groups in mixed-DNA samples, making them also suitable for prey species identification in dietary samples.