Prolonged Antimicrobial Activity of PMMA Bone Cement with Embedded Gentamicin-Releasing Silica Nanocarriers
Prolonged Antimicrobial Activity of PMMA Bone Cement with Embedded Gentamicin-Releasing Silica Nanocarriers
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DOI：
10.1021/acsabm.8b00752
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发表时间：
2019-05-20
影响因子：
4.7
通讯作者：
Prokopovich, Polina
中科院分区：
文献类型：
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作者：
Perni, Stefano;Caserta, Sergio;Prokopovich, Polina
经常使用含有抗生素的骨水泥来预防关节置换手术后的感染。我们开发了负载庆大霉素的二氧化硅纳米载体作为药物递送系统，分散在聚甲基丙烯酸甲酯（PMMA）骨水泥中，用于控制和延长抗生素从骨水泥中的释放，从而证明具有持久的抗菌活性。使用逐层自组装将庆大霉素沉积在藻酸盐层和二氧化硅纳米粒子上的两种不同的聚β-氨基酯之间。含有二氧化硅纳米载体的 PMMA 骨水泥中庆大霉素的释放持续约 30 天，而添加等量抗生素作为纯粉末（如商业制剂）时则为 6 天；此外，与商业制剂相比，含有释放的抗菌药物的介质能够在更长的时间内防止导致假体关节感染的多种细菌物种（目录菌株和临床分离株）的生长，从而证实了药物从载体中释放后的扩展抗菌特性。也没有观察到对人类成骨细胞的有害影响；此外，添加二氧化硅纳米载体时，骨水泥材料的特性，如固化时间、吸水率和机械性能不受影响。
Antibiotic laden bone cements are regularly employed to prevent infections after joint replacement surgeries. We have developed silica nanocarriers loaded with gentamicin as a drug delivery system to be dispersed in poly methyl-methacrylate (PMMA) bone cement for controlling and extending the release of the antibiotic from bone cements, thus proving a prolonged antimicrobial activity. Layer-by-layer self-assembly was used to deposit gentamicin between alginate layers and two different poly beta-amino esters on the silica nanoparticles. The release of gentamicin from PMMA bone cement containing silica nanocarriers continued for about 30 days compared to 6 days when the same amount of antibiotic was added as a pure powder (as in commercial formulations); moreover, the medium containing the released antimicrobial drug was capable of preventing the growth of numerous bacteria species responsible for prosthetic joint infections (both catalogue strains and clinical isolates) for longer periods of time than in the case of commercial formulations, thus confirming the extended antimicrobial properties of the drug once released from the carrier. No detrimental effects toward human osteoblasts were also observed; moreover, bone cement material characteristics such as curing time, water uptake, and mechanical properties were unaffected when the silica nanocarriers were added.